How Many list are show on the Purdue cytometry mail list for software sales...

F

FLOW CYTOMETER

Guest
...and are any hidden? Wake up people - times are changing - look at all
these new small companies trying to stick their noses in "our" field!


Wake up people - times are changing - look at all
these new small companies trying to stick their noses in "our" field!


Wake up people - times are changing - look at all
these new small companies trying to stick their noses in "our" field!

Purdue Cytometry Mailing List: Re: 2008 NW Regional Cytometry
Meeting, March 13 - 15 in Portland ... Technology, IntelliCyt,
Miltenyi Biotec, Partec, StemCell Technologies, Tree Star, Union
Biometrica, and Verity Software House. ...
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Meeting - Thanks!
... Purdue Cytometry Mailing List: New England Cytometry Users Group
Meeting - Thanks! ... C. Bruce Bagwell, MD, Ph.D. - Verity Software
House ...
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annual meeting reminder
... Purdue Cytometry Mailing List: New England Cytometry Users
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"Probability State Models: A ...
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... Purdue Cytometry Mailing List: New England Cytometry Users
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Document count: purdue (139318) cytometry (25468) mail (53709) list
(63448) 2007 (37553) verity (222) software (15216) sales (14402) 07
(21574) by (129519) thread (26375) purdue cytometry mail list 2007
verity software sales 07 by... (27962)
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Purdue Cytometry Mailing List: RE: DNA analysis software
... Purdue Cytometry Mailing List: RE: DNA analysis software ... J.
Herbert Technical Support Manager Verity Software House ...
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... vsh.com] > Sent: Wednesday, February 21, 2007 10:30 AM > To:
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Support Manager > Verity Software House ...
http://www.cyto.purdue.edu/hmarchiv/Current/0755.htm - 8.6KB
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... Purdue Cytometry Mailing List: Announcing User Forum at
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... From: Verity Software House <[email protected]> Date: Fri Sep 07
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- 17:30:59 EDT ... From: Verity Software House <[email protected]> Date:
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... Purdue Cytometry Mailing List: DNA analysis software ... we offer
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... Purdue Cytometry Mailing List: Cytometry software for linux ...
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Purdue Cytometry Mailing List: Re: FL2H in PI cell cycle analysis
... Purdue Cytometry Mailing List: Re: FL2H in PI cell cycle
analysis ... Next in thread: Verity Software House: "RE: FL2H in PI
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Purdue Cytometry Mailing List: New England Cytometry Users Group
annual meeting preliminary ...
... Purdue Cytometry Mailing List: New England Cytometry Users Group
annual meeting preliminary announcement ... up of speakers this year:
C. Bruce Bagwell, MD, Ph.D. - Verity Software House http://www.vsh.com/
Anne E. Carpenter, Ph.D. - MIT ...
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NW Regional ...
... Purdue Cytometry Mailing List: RE: Thanks to all for a successful
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... Purdue Cytometry Mailing List: RE: Diva 4.0 files ... Mark E.
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207-729-5443 ...
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Purdue Cytometry Mail List ENFORCES the SPAM rules!

[FONT=&quot]San Diego User's Group
From: Michele Lutz (ml...@vapop.ucsd.edu)
Date: Tue Sep 16 1997 - 15:45:04 EST

Next message: Gerhard Nebe-von-Caron: "Re: Flow cytometer location"
Previous message: Jeffrey M Scott: "..."
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]

--------------------------------------------------------------------------------

San Diego Area Flow Cytometry User's Group

VA San Diego Healthcare System
3350 La Jolla Village Dr.
San Diego, CA 92161

Multipurpose Room

Wednesday, September 24, 1997
6:00 to 9:00 PM

I. Highlights from Clinical Applications of Cytometry Annual Meeting
in
Charleston, South Carolina, August 1997
A. Overview of HIV Workshop
B. Brief discussion of emerging technologies

II. Presentation of interesting and/or problematic cases for
discussion by
user group members

Jeff Louie M.T.
Chief Technologist
Flow Cytometry Laboratory
University of Southern California
Chair of Flow Technologists Focus Group of the NIAID
Division of AIDS Flow Cytometry Advisory Committee

(program qualifies for 1.5 CEUs)

Meal provided by DAKO Corporation
--------------------------------------------------------------------------------
(please print or type clearly)

Name:_______________________________________________

Institution:_______________________________________________

Address:_______________________________________________

________________________________________________

________________________________________________

phone:_________________________ fax:____________________________

Case presentation
topic:_____________________________________________________

Please RSVP by September 22, 1997: will attend__________ will not
attend______

Eileen Bessent MT(ASCP), CLS or fax to (619)552-4370
VA Medical Center
Immunogentics Laboratory
3350 La Jolla Village Drive (113)
San Diego, CA 92161
(619)552-4379

--------------------------------------------------------------------------------

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--------------------------------------------------------------------------------
______________________________________________________________________________________

BUG Seminar correction
From: g...@MIT.EDU
Date: Fri May 30 1997 - 15:01:58 EST

Next message: Gary Durack: "Job Posting"
Previous message: Howard S. Mostowski 301-827-1807 FAX 301-496-7027:
"Clincical Trials T-Cell Lymph. G/D H/P"
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--------------------------------------------------------------------------------

Hi Everyone,

Because of a scheduling mistake beyond our control, we have changed
the
Boston Users Group meeting date from Wednesday June 11, 1997 to
Tuesday
June 10, 1997. Please advise anyone you know who was planning to
attend
and sorry for any inconveniences this may have caused.

Thank You,

Glenn Paradis
President Boston Users Group

Voice: (617) 253-6454
Fax: (617) 253-3714
Email: g...@mit.edu

++ Boston Users Group ++

MEETING ANNOUNCEMENT MEETING ANNOUNCEMENT MEETING
ANNOUNCEMENT
--------------------XXXXXXX----------------------
XXXXXXX----------------

The Boston Users Group for Cytometry (BUG) would like to invite all
it's
members and others to it's kick-off summer series cytometry symposium
on
June 10, 1997 . The Seminar will be held at Dana Farber Cancer
Institute
44 Binney St. Boston MA. in the Smith Family Room Dana 1820 from
1:00-2:30 PM.

Invited guest speaker will be Dr. Joern Schmitz of the Division of
Viral
Pathogenesis at Beth Israel Deaconess Medical Center/Harvard Medical
School who will be giving a lecture titled:

Employing Expression of the CD8 alpha/beta heterodimer
as a novel tool for the detection of discrete CD8+ T
lymphocyte subsets in PBL of HIV- or HIV+ individuals.

Dr Schmitz has had extensive "hands on" experience with all aspects
of
complex multicolor immunofluorescence flow cytometry and his talk
should
be of great interest to researchers interested in HIV as well as flow
cytometry in general .If you think you may be attending please
contact
either John Daley at 617-632-4571(john_da...@dfic.harvard.edu) or
Glenn
Paradis at 253-6454(g...@mit.edu). Lunch will be provided compliments
of
Coulter Cytometry.

Hope to see all fellow Bugs and flow(ers)
there!!

--------------------------------------------------------------------------------

Next message: Gary Durack: "Job Posting"
Previous message: Howard S. Mostowski 301-827-1807 FAX 301-496-7027:
"Clincical Trials T-Cell Lymph. G/D H/P"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]

--------------------------------------------------------------------------------

17:33:56 EST

______________________________________________________________________________________
:director::director::director::director::director:
From: "Gerhard Nebe-von-Caron" Unilever.com>[/FONT]
[FONT=&quot]>To: "Cytometry Mailing List flowcyt.cyto.purdue.edu>[/FONT]
[FONT=&quot]
\"jmsim...@IX.NETCOM.COM\"" flowcyt.cyto.purdue.edu>
(Return
requested)

- Hide quoted text -
- Show quoted text -[/FONT]
[FONT=&quot]>Subject: Re: Commercial websites (was Re: BDIS Home Page)
>Date: 09 Apr 1997 12:52:40 +0100[/FONT]
[FONT=&quot]> I also don't mind that bit of commercialism as it is quicker
> dumped in the e-mail than in the waste paper basket if I
> don't want it.[/FONT]
[FONT=&quot]> I still like to find a comprehensive list of commercial
> suppliers. The list between Purdue and Munich is pretty good
> but not necessarily up to date. Dako has for example their
> own home page and Biorad wasn't on them the last time
> either. Perhaps the best way to keep up to date is to allow
> the commercials to add their address to the pages themselves
> like Paul intends to do with the other site links.[/FONT]
[FONT=&quot]> Another thing that would be nice to have is some listing of
> 'useful information' like the compensation tutorial of Mark
> Roederer, the lin to log conversion from Dave Coder, the
> useful tips for winmdi users, how to clean laser brewster
> windows .... just to name a few. It also would be nice to
> get some of the commercial slides on flow principles (not
> pictures of gray or beige boxes on lab-benches) on the
> internet with the intention of free use. Perhaps even the
> flow tutorial from Coulter might be somewhere on the net.[/FONT]
[FONT=&quot]> Gerhard.Nebe-von-Ca...@unilever.com[/FONT]
[FONT=&quot]>______________________________ Reply Separator[/FONT]
[FONT=&quot]
_________________________________

- Hide quoted text -:crazy::crazy::crazy::crazy::crazy::crazy:
- Show quoted text -[/FONT]
[FONT=&quot]>Subject: Commercial websites (was Re: BDIS Home Page)
>Author: jmsim...@IX.NETCOM.COM at INTERNET
>Date: 08/04/97 23:43[/FONT]
[FONT=&quot]>Thanks, Jeff, for letting us know about the Bio-Rad website.[/FONT]
[FONT=&quot]>One doesn't always have the time to check on all of the FCM related
>sites and update our links.[/FONT]
[FONT=&quot]>You're correct to point out that commercialism should be kept at a
>minimum on this list, but short notices regarding useful info, I
>believe, are OK.[/FONT]
[FONT=&quot]>(Sorry if I stepped on anyone's toes re: my BDIS homepage post; just
>wanted to get the info out and, no, I don't get any commission) :)[/FONT]
[FONT=&quot]>Ciao,
>Jim[/FONT]
[FONT=&quot]
The Coulter Corporation's international Web Site is to be found at,
coulter.com

Alan D. Logan
Coulter Electronics Pty. Ltd.
Sydney
Australia

________________________________________
• Next message: Jorg Ueckert: "Re: Wanted: Supplier for DiIC1(5)"
• Previous message: Susan Rice: "Cell Sorter for Sale":nono::nono:
• Maybe in reply to: James M. Simone: "Commercial websites (was
Re:
BDIS Home Page)"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:33:56 EST

Re: Commercial websites (was Re: BDIS Home Page)
From: Gerhard Nebe-von-Caron (Gerhard.Nebe-von-Ca...@unilever.com)
Date: Wed Apr 09 1997 - 06:52:40 EST

Next message: Mike Keeney: "SHEEP TNF MOAB'S"
Previous message: Pizzo,Eugene: "RE: anti-HLA mAb"
Maybe in reply to: James M. Simone: "Commercial websites (was Re: BDIS
Home Page)"
Next in thread: Alan D. Logan: "Re: Commercial websites (was Re: BDIS
Home Page)"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]

--------------------------------------------------------------------------------

I also don't mind that bit of commercialism as it is:evil:
quicker
dumped in the e-mail than in the waste paper basket if I
don't want it.

I still like to find a comprehensive list of commercial
suppliers. The list between Purdue and Munich is pretty
good
but not necessarily up to date. Dako has for example their
own home page and Biorad wasn't on them the last time
either. Perhaps the best way to keep up to date is to
allow
the commercials to add their address to the pages
themselves
like Paul intends to do with the other site links.

Another thing that would be nice to have is some listing of
'useful information' like the compensation tutorial of Mark
Roederer, the lin to log conversion from Dave Coder, the:gossip:
useful tips for winmdi users, how to clean laser brewster
windows .... just to name a few. It also would be nice to
get some of the commercial slides on flow principles (not
pictures of gray or beige boxes on lab-benches) on the
internet with the intention of free use. Perhaps even the
flow tutorial from Coulter might be somewhere on the net.

Gerhard.Nebe-von-Ca...@unilever.com

______________________________ Reply Separator
_________________________________
Subject: Commercial websites (was Re: BDIS Home Page)
Author: jmsim...@IX.NETCOM.COM at INTERNET
Date: 08/04/97 23:43

Thanks, Jeff, for letting us know about the Bio-Rad website.:spy:

One doesn't always have the time to check on all of the FCM related
sites and update our links.

You're correct to point out that commercialism should be kept at a
minimum on this list, but short notices regarding useful info, I
believe, are OK.

(Sorry if I stepped on anyone's toes re: my BDIS homepage post; just
wanted to get the info out and, no, I don't get any commission) :)

Ciao,
Jim:photo:

--------------------------------------------------------------------------------

Next message: Mike Keeney: "SHEEP TNF MOAB'S"
Previous message: Pizzo,Eugene: "RE: anti-HLA mAb"
Maybe in reply to: James M. Simone: "Commercial websites (was Re: BDIS
Home Page)"
Next in thread: Alan D. Logan: "Re: Commercial websites (was Re: BDIS
Home Page)"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]

--------------------------------------------------------------------------------

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:33:56 EST

Re: Commercial websites (was Re: BDIS Home Page)
From: Alan D. Logan (a.lo...@s054.aone.net.au)
Date: Thu Apr 10 1997 - 03:31:01 EST

Next message: Jorg Ueckert: "Re: Wanted: Supplier for DiIC1(5)"
Previous message: Susan Rice: "Cell Sorter for Sale"
Maybe in reply to: James M. Simone: "Commercial websites (was Re: BDIS
Home Page)"

Next message: Calman Prussin: "RE: data display"
• Previous message: John Mill: "Flow Services"
• Next in thread: Calman Prussin: "RE: data display"
• Maybe reply: Calman Prussin: "RE: data display"
• Maybe reply: David L. Haviland, Ph.D.: "RE: data display"
• Maybe reply: Donnenberg, Albert: "RE: data display"
• Maybe reply: Gib Otten: "RE: data display"
• Maybe reply: Bob Ashcroft: "RE: data display"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:34:03 EST
Flow Services
From: John Mill (jaguar19@ix.netcom.com)
Date: Tue Sep 30 1997 - 08:46:23 EST
• Next message: Houston, Jim : "RE: data display"
• Previous message: JANET FISHER: "32D mouse cells"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
Greetings-:high5::attention::attention:

ALTechnologies is a biotech company with laboratories located in
Rockville,
Maryland. We have a need for flow cytometry services. If you offer
such
services please respond via email. We wish to develop a relationship
with
such a company, and work out all of the costs, transportation, and
other
details priot to generating a contract. Thank you in advance for your
time.

John Mill

--
________________________
Dr. John Mill (mill@ALTechnologies.com)
ALTechnologies: http://www.ALTechnologies.com
Cytogenetic and FISH Probes and Products
________________________________________
• Next message: Houston, Jim : "RE: data display"
• Previous message: JANET FISHER: "32D mouse cells"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
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________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:34:03 EST

Commercial websites (was Re: BDIS Home Page)
From: James M. Simone (jmsim...@ix.netcom.com)
Date: Mon Apr 07 1997 - 20:25:54 EST

Next message: McGilp, Rob: "RE: Avidin conjugation of antibody"
Previous message: Larry Arnold: "Re: Yeast Cell Cycle Method"
Next in thread: Gerhard Nebe-von-Caron: "Re: Commercial websites (was
Re: BDIS Home Page)"
Maybe reply: Gerhard Nebe-von-Caron: "Re: Commercial websites (was Re:
BDIS Home Page)"
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Home Page)"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]

--------------------------------------------------------------------------------

Thanks, Jeff, for letting us know about the Bio-Rad website.:biggrin1:

One doesn't always have the time to check on all of the FCM related
sites and update our links.

You're correct to point out that commercialism should be kept at a
minimum on this list, but short notices regarding useful info, I
believe, are OK.

(Sorry if I stepped on anyone's toes re: my BDIS homepage post; just
wanted to get the info out and, no, I don't get any commission) :)

Ciao,
Jim

--------------------------------------------------------------------------------

Next message: McGilp, Rob: "RE: Avidin conjugation of antibody"
Previous message: Larry Arnold: "Re: Yeast Cell Cycle Method"
Next in thread: Gerhard Nebe-von-Caron: "Re: Commercial websites (was
Re: BDIS Home Page)"
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BDIS Home Page)"
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Home Page)"
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--------------------------------------------------------------------------------

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:33:56 EST

Re: BDIS Home Page
From: James M. Simone (jmsim...@ix.netcom.com)
Date: Mon Mar 31 1997 - 15:14:43 EST

Next message: Oleg S. Vasilyev: "Re: normal levels of HLA-DR on CD4"
Previous message: adur...@notes.mdacc.tmc.edu: "Re: Thy1/CD34
staining"
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--------------------------------------------------------------------------------

If you havn't seen it yet, point your browser to the new BDIS website:

http://www.bdfacs.com/:gossip:

Although the site is still under construction, one can already obtain
a
wealth of information on various topics including company info,
products, technical support, etc.

Moreover, the pages load quickly and are nicely layed out so you can
efficiently get the information you need.

Check it out!

Jim

--------------------------------------------------------------------------------

Next message: Oleg S. Vasilyev: "Re: normal levels of HLA-DR on CD4"
Previous message: adur...@notes.mdacc.tmc.edu: "Re: Thy1/CD34
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--------------------------------------------------------------------------------

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Re: BDIS Home Page
From: Joseph Chmielowski (ch...@ix.netcom.com)
Date: Mon Mar 31 1997 - 19:35:50 EST

Next message: Nicholson, Janet: "normal levels of HLA-DR on CD4"
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--------------------------------------------------------------------------------

- Hide quoted text -
- Show quoted text -
James M. Simone wrote:[/FONT]
[FONT=&quot]> If you havn't seen it yet, point your browser to the new BDIS website:[/FONT]
[FONT=&quot]> http://www.bdfacs.com/:spy:[/FONT]
[FONT=&quot]> Although the site is still under construction, one can already obtain a
> wealth of information on various topics including company info,
> products, technical support, etc.[/FONT]
[FONT=&quot]> Moreover, the pages load quickly and are nicely layed out so you can
> efficiently get the information you need.[/FONT]
[FONT=&quot]> Check it out![/FONT]
[FONT=&quot]> Jim[/FONT]
[FONT=&quot]
Also,

Check out the Coulter Web Page at

http://www.coulter.com:high5:

It is a wealth of information and even has an on-line catalog!

Joe.

--------------------------------------------------------------------------------

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CAC Commercial Workshop:nono:
From: J.Paul Robinson (j...@flowcyt.cyto.purdue.edu)
Date: Wed Jul 29 1998 - 22:09:42 EST

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I have been requested to post this notice about a workshop coming up
at
CAC. Here it is.
Paul:gossip:

- Hide quoted text -
- Show quoted text -[/FONT]
[FONT=&quot]> * * * * * * * * * * * * * * * * * * * * * * * * * * * *[/FONT]
[FONT=&quot]> A N N O U N C E M E N T:crazy:[/FONT]
[FONT=&quot]> * * * * * * * * * * * * * * * * * * * * * * * * * * * *[/FONT]
[FONT=&quot]> Scientific Tutorial
> at the
> 13th Annual Meeting
> Clinical Applications of Cytometry[/FONT]
[FONT=&quot]> The Hematopathological Model to Hematopoietic Malignancies:
> Applications of Novel Technologies[/FONT]
[FONT=&quot]> Dr. Jean Lopategui, Medical Director and
> Horacio Vall, Flow Cytometry Supervisor
> IMPATH Laboratories, Los Angeles CA[/FONT]
[FONT=&quot]> Wednesday, August 19, 1998
> 3:45-5:45pm
> Charleston Place
> Charleston, South Carolina[/FONT]
[FONT=&quot]> Sponsored by Coulter Corporation[/FONT]
[FONT=&quot]:fish2:
----------------------------------------------------------------------------
----
This message was posted on the Purdue Cytometry Discussion Group by

--------------------------------------------------------------------------------

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Publication quality graphs:crazy:
From: Mario Roederer (Roede...@Darwin.Stanford.EDU)
Date: Wed Sep 24 1997 - 16:29:44 EST
• Next message: Mario Roederer: "Contour plots & smoothing: rights
and
wrongs"
• Previous message: Ronald Rabin: "Cross-linked anti-IgM"
• In reply to: david.mcfarl...@mcmail.vanderbilt.edu: "Re:
graphics
presentation summary"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________[/FONT]
[FONT=&quot]> The Mac QuickdrawPICT 72 dpi limitation on resolution is
> the real problem using the Mac + CellQuest -> desktop
> publishing applications.[/FONT]
[FONT=&quot]
This is not a general problem of the MAC, it is simply an hurdle that
most
applications don't bother to cross. Specifically, it is no problem
with FlowJo,
which can read Cellquest-generated FCS files (as well as any other
program's FCS
files). FlowJo generates graphics in PICT format, meaning that the
graphs that
you copy to your publication programs are NOT bitmaps (unless you
desire
this--it is a preference). This means that, for instance, when you
copy a
contour plot to Canvas, each contour is a separate polygon that can be
individual colorized, or have the pen pattern changed, etc. Also,
when you copy
histograms, you can easily set the pen patterns--for instance, dashed
lines,
different fill patterns etc. You can also easily resize graphs
without losing
any resolution.

For an example of the kinds of graphs that can be generated on a
Macintosh, see
"http://www.treestar.com/flowjo/documentation/graphwindow/
graphexamples.html".:director:

mr[/FONT]
 
Purdue Cytometry mail list EXPOSED

Which Government agency would Investigate the Purdue Cytometry Mail
List and Isac Congress/ Who do I notify to Enforce the laws and make a
Complaint about the Purdue Cytometry Mail List and the PRESIDENT of
Isac Congress?

The Entity I make the Complaint to should be able to Investigate BD,
and the rest of the software Companies since our software was sent
back DYSTROYED and we are concerned about our CODE. Since Mr.
Gunderman explained we were ahead of the times. 40 PARAMETERS BY 10
MILLION EVENTS. FCS 3.0 NO CONFUSING MENU BAR. 24-48,000 FILES PER
HOUR.

J Paul Robinson President of Isac CONGRESS and Head of Purdue
Cytometry Mail List called our Corporation Kanecki Associates Inc.
Scammers Internationally through the Purdue cytometry Mail List and
Communicated to Pass the Word through ISU Professor Larry Farell after
we sent a message informing Robert Murphy President elect about our
Corporations break through in NEW TECHNOLOGY.

The LETTER was not sent to J Paul Robinson but some how he intercepted
it. Regardless the Details of the Flow Cytometry Software for only
$150.00 for student..NO License Fees:usa:


All flow Cytometry Software Companies Charge Large License fees . We
thought this would be a great Idea for STUDENTS and the rest of the
world. Obviously, Mr. Robinson DID NOT Like this message responding
to Steve with Concern that the LETTER may have gotten to HIS MAIL LIST
stating that the Information sent for Consideration was JUNK MAIL.

After I sent Messages back to Mr. Robinson he still insisted that it
was Junk mail.:spider:
I have Evidence that Could make a great case for Discrimination,
Filtering,Collusion, and Much More ESPECIALL SINCE there is only 5
software Companies ALL ON THE MAIL LIST and all Developed thier own
FlowCytometry software while on the LIST.


This is a LETTER FROM THE MAIL LIST TO SUPPORT THE RULES!


:hurt:
Recent FlowJo announcementFrom: Steve Kelley
(SKELLEY@flowcyt.cyto.purdue.edu)
Date: Wed Dec 30 1998 - 06:13:04 EST

Next message: Steve Kelley: "Possible minor disruption"
Previous message: Mark A. Corio: "Chemdex no help..."
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[ attachment ]
I know that many people on the mailing list are adamantly opposed to
anything that even looks a little commercial, and to try to forestall
any possible

complaints about the FlowJo announcement:hail:
, I'll explain what

I'd like to see. In my opinion, announcements about new products, and
product updates are completely appropriate as long as they aren't
abused.
I've never wanted to specifically encourage that, because I really
don't want to be put into a position of having to decide whether a
message is an

"announcement"
or an

"advertisement

 . The companies involved in our cytometry community have always been
extremely good 'citizens' as far as I can tell.



We have representatives of many companies on the list, and they have
always had the power to make my life miserable, and the list no more
than junk mail. Instead, they have helped build this mailing list
into one of the most useful around, through their contributions :first:

and
responses. I'm not going to try to set specific rules about what
people can say about their own products, and when they can say it.


I'll just ask that everyone continue to show restraint; that the
commercial representatives ask themselves before they submit an
announcement whether they'd mind seeing every other company in the
business sending the same message they are about to, and that the


non-
commercial (and anti-commercial) people accept discreet announcements
as simple information, and continue the sometimes brisk discussions
about problems and benefits of particular products, alongside the
purely scientific (and occasionally purely entertaining) conversation.
Steve Steve Kelley
kelley@flowcyt.cyto.purdue.edu
Purdue University Cytometry Laboratories (765) 494-0757 --
voiceB050 Hansen LSRB, Purdue University (765) 494-0517 --
faxWest Lafayette, Indiana, 47907 -- End --

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17:35:29 EST
:caked:
After these Conversations with Mr. Robinson, Bill Gunderman from BD
refused to return our phone calls. After 3months he finally took my
Call at night. At this time he explained we were there COMPETITION.

Nothing seemed to make sense..all the FORUMS I joined banned me.
Anytime I mentioned our software it got DELETED!:lock1:


Then when I started asking Questions on Yahoo ie Why do companies
charge license some one sent me a link to my personal email that
exposed the mail list. When I CLICKED on the link it took me through a
BACK ROUTE so I was able to download all the Purdue Cytometry Mail
List ARCHIVES SINCE 1992.:painkiller:

Soooo much to read but It was easy to see how everyone in the User
groups, software companies, forums. science advisory board,ect were on
the PURDUE CYTOMETRY MAIL LIST and were a part of ISAC.

J. Paul Robinson
<jpr_at_flowcyt.cyto.purdue.edu>

ViewMonday, November 19, 2007 2:14:38 PM

To:Larry Farrell <farrlarr_at_isu.edu>; mitchell haynes
<buybroker_at_yahoo.com>;
skelley_at_flowcyt.cyto.purdue.edu; Bartek Rajwa
rajwa_at_flowcyt.cyto.purdue.edu

Larry

Sorry you are experiencing a problem:pound:

>From the header - This is apparently coming from a Mitchel Haynes
mitchell haynes <buybroker_at_yahoo.com> - is this correct?

I initially thought messages coming from this person were a scam and

challenged them when they started posting to our list as well. I had

someone do a check on them. it is apparent that they have written
some

software and they think its ants-pants. They have tried to post
things on the Purdue list , they have used every possible website and email

discussion group to get cross listed to boost their ratings on
Google.

Basically I view their behavior as very tenuous and from the message
you

sent me, it appears that it is not appropriate to do what they are
doing...

I have taken the following action::humble:

Steve Kelley has been instructed to remove their email and any
postings on the Purdue list. They are now banned

2. I am going to Copying Bartek Rajwa the editor of the ISAC site to

beware of them:painkiller:

3. I am contacting Google and other sites to let them know that these

people are self-propagating links.:rant:

4. If I see any message that in any way impunes Purdue or our

reputation, I will go after them with every possible legal recourse
at

my disposal.

We will not allow our reputation to suffer because of

commercial abuse.:painkiller:

5. You should ban this address, and basically indicate to your
members

that this is a scam. While they claim to be legitimate, they are
acting

exactly as any scam artist...



I already told them that they were
acting

as scammers and they got upset with me..

.well, if they don't desist,:painkiller:

they will find out how much influence we actually have...:humble:


I will check the message:hurt:
> Subject: flow cytometry software will you trust Purdue..Lets play
MONOPOLY
> Date: Mon, 19 Nov 2007 09:38:56 -0800 (PST)
> Organization: http://groups.google.com
and see if this is actually libelous - it appears to be - and if it
is,

I will close this person down really, really fast!!! (Steve please
check

out this message and make a copy):focus:



Larry, we have about 3000 people on the Purdue discussion list which
is

primarily about cytometry - and a lot of flow cytometry -

to join,people actually have to go through me,:hail:

and we review every person

who

comes on.
:gossip:
I am sorry you are experiencing this problem, and we are happy to
help

in any way we can.

regards

paul Robinson:painkiller:

Professor, Purdue
GLarry Farrell wrote::violin:
> Someone has apparently decided to post the following, and a huge number
> of related messages, to misc.health.aids. I have asked that person,
on
> several occasions, not to post this material into that newsgroup and
> have been ignored. My reasons for that request are two-fold: (1)
> Misc.health.aids is an HIV/AIDS group so the material is decidedly off
> topic, and (2) Some of these messages contain reference to a site from
> which cytometry software can be purchased and commercial solicitations
> of any sort are expressly forbidden by the group's charter. Is there
> any way you can intervene in this issue and stop the *many* posting
of
> this and related cytometry messages in misc.health.aids?
NO READ WHAT WAS SENT TO ROBERT MURPHY NOT J PAUL ROBINSON



Dear Robert Murphy

NEW
FLOWCYTOMETRY SOFTWARE FOR ALL BD and Beckman/Coulter Flow Cytometers
CAN
PROCESS 24,000-42,000 SAMPLES PER HOUR. FLOW CYTOMETRY FCS CYTO PRO
QUICK FACS Kanecki Associates - The Future of Software Technology and World Leader in Intelligent Thinking Systems Management Government Military Intellectual Property

Increased quality and productivity. With 10,000,000 event files, you
can process 24,000 samples/hour, and maintain quality up to Sigma 5
or
better. Compare this to having your research technologist performing
only 100 samples/hour analysis.
Increased laboratory utilization by 3X because you can perform the
analysis off-lab and free laboratory time for reading samples. This
was achieved when I developed the program, and we had a program
project grant from 1992 to 1998 of $8M.
Works with FCS 3.0 in all data modes as floating point, integer*4,
and
ASCII.
Works with BD and Beckman/Coulter Flow Cytometers and Cell Sorters
Backwards compatible with FCS 2.0 files and Flow Cytometers and Cell
Sorters.
Can read FCS 3.0 files up to 10M events with 20 parameters. Easy to
Use, three step process. Load initial file, set gate, specify file
list to process. That's it.
Collaboration tools to allow you to cut and paste image results to
results.
Statistical analysis results imprinted on histogram plots directly as
mean, mode, and median with the ability to present results in log
mode
or linear mode, depending on the detector used. Plain vanilla coding
for easy update and maintenance to allow for the greatest user and
software quality.
One time purchase fee, no yearly renewal fees as with others. Proven
tract record in FACS, Fluorescent Activated Cell Sorter Laboratory.
The laboratory was rated the best laboratory in the Midwest USA in
1990.

This application is designed for large-scale fluorescent activated
cell sorter
analysis. The program can read up to FCS 3.0 files and has been
tested
to run on Becton Dickinson and CoulterOrtho based flow cytometers and
cell sorters. The main advantage of this program is that you can have
the computer perform the analysis for you after you have selected the
region to analyze. The result is that up to 24,000 samples per hour
can be analyzed on a 1.4 GHz speed computer. This program is designed
for researcher and technologist use. It uses rectangular gating, and
is intuitive to use. To use this program, the FCS must have the
extension, *.bin as "54203023.bin" as an example. The *.bin extension
is what the computer uses to locate the files on the computer.

THANK YOU FOR YOU TIME MITCHELL HAYNES VP
SALES KANECKI
ASSOCIATES 832-347-1669



THIS WAS J PAUL ROBINSON RESPONSE TO THE INFORMATION SENT Re: Fw: NEW
FLOWCYTOMETRY SOFTWARE FOR ALL BD and Beckman/Coulter Flow Cytometers
CAN PROCESS 24,000 SAMPLES PER HOUR...Standard Header|Full Message
View J. Paul Robinson J. Paul Robinson ... ViewFriday, September 28,
2007 9:37:32 AM To:mitchell haynes Cc:david_at_kanecki2.com;
skelley_at_flowcyt.cyto.purdue.edu

:humble:
Steve
what is this email - it came to me with Bob Murphy's name associated
with it. It seems to be anadvertisement, this junk mail, and it
seems
to have been modified by you ...:jaw:

So I guess I am confused. was this sent to the list,

or
do you have anydetails about it -

i am concerned about these junk messages going out
to
our members,:sorry:

- if they are using our lists, I will deal with them
appropriately,

but I am not happy about this :nono:

- any info you can give
me
appreciated

thanks

paul
WHILE BEING SHOCKED BY THIS MAIL...MITCHELL HAYNES VP.KANECKI
ASSOCIATES INC....WROTE BACK : mitchell haynes
<buybroker_at_yahoo.com>

> To: skelley_at_flowcyt.cyto.purdue.edu
> Cc: skelley_at_flowcyt.cyto.purdue.edu
> Sent: Friday, September 28, 2007 11:39:02 AM
> Subject: NOT A JUNCK MAIL STEVE WAS JUST FOWARDING INFORMATION TO PROPER
> CHANNELS
>


Dear Paul,
> I recieved your responce to the email I sent. Please understand it is not
> junkmail but a update on new technology that will inhance all
> flowcytometers..It is currently being evaluated by BD who request for this
> software to be developed directly by our corporation.
> It was simply sent as an announcement for you concideration.
> The software is demonstrates precision and a higer processing rate than
> every existing software today.
> If you have any questions please call I provided my phone number with the
> email. I understand institutions of your caliber is always looking for new
> technology. Futhermoore this is the only software in the world that works
> for every platform on one peice of software
> Thank your for you time and have a great day.
> Please do not blame Steve for send the information to the proper channels
> I would think you would be upset if he did not foward important infomation
> that pertains to furthering cytometry breakthroughs.
> If you would like us to send information to another address that won't
> interfer please foward it to me and I will make sure that there are no
> more misunderstandings.
Mitchell Haynes

From: "jpr_at_flowcyt.cyto.purdue.edu"
<jpr_at_flowcyt.cyto.purdue.edu>

To: mitchell haynes <buybroker_at_yahoo.com>

Cc: jpr_at_flowcyt.cyto.purdue.edu

Sent: Friday, September 28, 2007 2:26:51 PM
:focus:
Subject: Re: Fw: NOT A JUNCK MAIL STEVE WAS JUST FOWARDING
INFORMATION
TO
PROPER CHANNELS Sorry, I think it is junk mail

regards

paul robinson

On Nov 30, 7:23 pm, Larry Farrell isu.edu> wrote:

- Hide quoted text -
- Show quoted text -

> Mitch Haynes wrote:
> Mitch Haynes wrote:
>> On Nov 30, 2:01 pm, Larry Farrell isu.edu> wrote:
>>> Mitch Haynes wrote:
>>>> Why do you continue to post cytometry materials in misc.health.aids?
>>>> That is an HIV/AIDS newsgroup and your material is completely off
>>>> topic. No one in the group is interested.
>>>> --
>>> [Multiple copies snipped]
>>> Very interesting. I got a "Mail Undeliverable" notice when I sent
>>> this directly to Mr. Haynes but he turns around and posts multiple
>>> copies of a personal e-mail message into a newsgroup. Extremely poor
>>> Netiquette (although it is just a further example of the poor
>>> Netiquette displayed by the issue about which I tried to contact him).
>>> Exactly what is it that you are trying to accomplish, Mr. Haynes?
>>> --


>>> Larry D. Farrell, Ph.D.
>>> Professor of Microbiology
>>> Idaho State University
>>> --
>>> Posted via a free Usenet account fromhttp://www.teranews.com
>> PLEASE PROVIDE PROOF OF UNDELIVERALBE MAIL NOTICE...I GOT YOUR MAIL SO DID PAUL ROBINSON....WHY DON'T YOU CARE....?
>> THAT IS THE PROBLEM....ONLY GOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOGLE WILL
>> PROVIDE THE INFORMATION TO THE WORLD...:angel:
>> ISSUE....WHY DID YOU NOTIFY J PAUL ROBINSON ABOUT POST?
>> IT IS GOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOLES WEBSITE........

>> I BELIEVE THE WORLD NEEDS TO KNOW....YOUR IMPORTANT POST ARE NOT
>> BLOCKED....SO POST WHAT YOU LIKE....
>> GLAD IM NOT YOUR STUDENT TOO

>> I GOT YOUR MAIL.....

 > --
> Larry D. Farrell, Ph.D.
> Professor of Microbiology
> Idaho State University> On Nov 30, 2:01 pm, Larry Farrell
> isu.edu> wrote:
>>> Mitch Haynes wrote:
>>>> Why do you continue to post cytometry materials in misc.health.aids?
>>>> That is an HIV/AIDS newsgroup and your material is completely off
>>>> topic. No one in the group is interested.
>>>> --
>>> [Multiple copies snipped]
>>> Very interesting. I got a "Mail Undeliverable" notice when I sent
>>> this directly to Mr. Haynes but he turns around and posts multiple
>>> copies of a personal e-mail message into a newsgroup. Extremely poor
>>> Netiquette (although it is just a further example of the poor
>>> Netiquette displayed by the issue about which I tried to contact him).
>>> Exactly what is it that you are trying to accomplish, Mr. Haynes?
>>> --
>>> Larry D. Farrell, Ph.D.
>>> Professor of Microbiology
>>> Idaho State University
:gossip:

 Paul Robinson was *not* informed of my message to you. What makes
you
> think he was?


> I deleted the Undeliverable Mail notice as soon as it was received,
> assuming that it was a dead issue since you would not have received the
> message. And then, lo and behold, you actually did. Something is fishy!
> Why do I care? Because you are flooding a normally useful newsgroup
> with off-topic material, wasting bandwidth and turning off people who
> might read the group. Your behavior in this group certainly does not
> reflect well on you or on Kanecki Associates, the company for which you
> apparently work as Marketing Director.
> You have no idea how glad I am that you aren't one of my students!!
> --
> Larry D. Farrell, Ph.D.
> Professor of Microbiology
> Idaho State University

After sending a letter to his Corporate

It was sent a Link to the Purdue Cytometry Mail List which EXPOSED how
software was developed over the years and the VENDORS on the LIST were
in the Software Business.
Rules of the Purdue Cytometry Mail list allows for NEW TECHNOLOGY
especially if it EXPANDS the FIELD in which ISAC is concerned.

We developed software for BD due to a request by BILL GUNDERMAN. We
made the Software to PROCESS FCS 3.0 with all 128 Permeations calling
our announcement that was Sent to Robert Murphy President Elect of
Isac also at Purdue. He transfered from Purdue AFTER this. He will be
the next ISAC president.
 
[FONT=Courier, Monospaced]What point on Purdue Cytometry Mail List is FCS PRESS Making about the
Flow Jo marketing?


FCSPress Training Sessions?

From: Ray Hicks (r...@fcspress.com)
Date: Thu Oct 11 2001 - 19:42:36 EST
Next message: Laird Bloom: "RE: Multiple plate loader?"
Previous message: flow: "RE: HELP! How to clean the sample injection
tube?"

In reply to: Jennifer Wilshire: "FlowJo Training Sessions (Midwest)"
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--------------------------------------------------------------------------------
Most of you will find FCSPress so easy to use that you won't need
training
sessions. You can download a copy to try it out for yourselves at
http://www.fcspress.com/html/FCSPArea.html
where you'll also find documentation and details of a special offer.
If you find you DO need some assistance using FCSPress, I'm planning
on
staying in Cambridge for quite some time, and I'd be happy to answer
any of
your queries via e-mail at supp...@fcspress.com
Cheers,
Ray
--
e-mail mailto:R...@FCSPress.com
Web http://www.FCSPress.com
Tel +44 797 453 8647
+44 1223 871081
Fax +44 870 7408595
--------------------------------------------------------------------------- -----
Next message: Laird Bloom: "RE: Multiple plate loader?"
Previous message: flow: "RE: HELP! How to clean the sample injection
tube?"
In reply to: Jennifer Wilshire: "FlowJo Training Sessions (Midwest)"
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This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:40:27 EST
FlowJo Training Sessions (Midwest)
From: Jennifer Wilshire (GoFlo...@yahoo.com)
Date: Thu Oct 11 2001 - 06:20:11 EST
Next message: Richard Grenfell: "Multiple plate loader?"
Previous message: William King: "Searching for pig anti-CD34"
Next in thread: Ray Hicks: "FCSPress Training Sessions?"
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[ attachment ]

I will be in Wisconsin, Minnesota, and Iowa offering FlowJo seminars
and training sessions next week. All of the hosting sites have
graciously opened the sessions to members of the Cytometry list.
Times
and locations are listed below. Thanks, Jennifer Wilshire FLOWJO
SEMINARS ______________________________________ Monday, October 15th
University Hospitals Clinical Sciences Center 600 Highland Ave,
Madison WI Room K4/418 10:00 AM
______________________________________
Tuesday, October 16th University of Minnesota Cancer Center 425 E.
River Road Room 450 9:00 AM ¬ Introduction to FlowJo 10:30 AM ¬
Advanced Topics _______________________________________ Tuesday,
October 16th University of Minnesota, St. Paul Building AS/VM Room
295M 2:00 PM _______________________________________ Friday, October
19th University of Iowa South East 310 General Hospital Bean
Conference Room 10:00 AM _______________________________________ If
you are interested in hosting a FlowJo seminar, please let me know.
Upcoming trips are in the planning stages for: -NJ, PA, DC -Florida.
-
Boston (home) Jennifer Wilshire, Ph.D. Application Scientist
GoFlo...@Yahoo.com Tree Star, Inc. www.flowjo.com
Next message: Richard Grenfell: "Multiple plate loader?"
Previous message: William King: "Searching for pig anti-CD34"
Next in thread: Ray Hicks: "FCSPress Training Sessions?"
Reply: Ray Hicks: "FCSPress Training Sessions?"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
-------------------------------------------------------------------------------- [/FONT]
 
What is Meant By Blantent Advertising On the Purdue Cytometry Mail List?

This message: [ Message body ] [ More options ]
> Related messages: [ Next message ] [ Previous message ]
> From: VSH - Tech Support <[email protected]>
> Date: Wed Jun 30 2004 - 12:08:14 EST
> David
> At the risk of sounding commercial, WinList can do what you want. I
> have
> attached a few pictures showing the WinList Region Array feature.
> With this
> you can tell the program exactly how you want the regions created and
> how
> many. If you would like to try it for yourself you can download a
> trial
> version from our web sitewww.vsh.com.
> Please let me know if you have any questions.
> Best regards
> Don
> Donald J. Herbert
> Technical Support Manager
> Verity Software House, Inc.
> PO Box 247
> 45A Augusta Road
> Topsham, ME, USA 04086
> Phone: (207) 729-6767 ext.190
> Fax: (207) 729-5443
> email: [email protected]
> web:Verity Software House
  • 1 week ago
Additional Details

1 week ago
anti) Commercial Posting:)
> This message: [ Message body ] [ More options ]
> Related messages: [ Next message ] [ Previous message ]
> From: Ray Hicks <[email protected]>
> Date: Wed Oct 13 2004 - 08:43:13 EST
> Just a quick nopte to let people know that FCSPress is now shareware
> (no time restrictions or feature reductions for free use, though if
> you feel like like paying for it you can using paypal). It also now
> works with Diva export files and runs ok under classic on OS 10.2 and
> 10.3. I've discontinued FCS Assistant, which has fallen behind
> FCSPress remarkably, and which failed to run as shareware on some
> systems (it insisted you buy it or refused to run - FCSPress doesn't
> even have a nag screen now - pesky things).
> FCSPress is available fromwww.fcspress.com
> Enjoy!
> Ray
1 week ago
From: VSH Tech Support [mailto:[email protected]]
> > Sent: Wednesday, February 21, 2007 10:30 AM
> > To: Cytometry Mailing List
> > Subject: RE: DNA analysis software
> > Hello Ibtissam,
> > ModFit LT, for PC or Mac, has advanced modeling capability for
> > research applications in DNA cell cycle analysis. You may use any of
> > the model templates the program offers, or create your own models for
> > non-traditional analysis, including non-mammalian DNA cell cycle
> > studies. ModFit LT can be linked to our WinList program to provide a
> > complete cell cycle analysis on any number of sub-populations with a
> > single click of a button.
> > For an overview, visithttp://www.vsh.com/products%a0.
> > Best regards,
> > Don
> > Donald J. Herbert
> > Technical Support Manager
> > Verity Software House
> >
1 week ago
FW: FlowJo Seminar for Los Angeles Basin
From: Barsky, Lora ([email protected])
Date: Tue Mar 20 2001 - 14:28:04 EST

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--------------------------------------...

I'm not sure if there is a Los Angeles Basin users
group,
but thought I could advertise here to interested parties.
Thanks,

Lora Barsky - Lead Operator
Research Immunology/BMT FACS Core
Childrens Hospital Los Angeles
1 week ago
Re: Software
From: Ray Hicks ([email protected])
Date: Fri Jul 04 1997 - 04:15:27 EST

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--------------------------------------...

Hi Vincent,

I'm developing an analysis program, based on FCS Assistant, called
FCSPress. So far it's quite rudimentary and only allows you to plot
histograms. It doesn't do gating or analysis yet. And there is little
support for the edit menu functions (cut, copy, paste, undo). Unlike
most
cytometry programs available on the mac, it allows you to open a data
file
and optionally manipulate its contents, graphs are plotted from the
active
data window into graph windows,
1 week ago
Document count: purdue (139318) cytometry (25468) mail (53709) list
(63448) 2007 (37553) verity (222) software (15216) sales (14402) 07
(21574) by (129519) thread (26375) purdue cytometry mail list 2007
verity software sales 07 by... (27962) about 187244 results found, top
500 sorted by relevance score using date hide summaries group by
location spacer 1-10 next Purdue Cytometry Mailing List: RE: DNA
analysis software ... Purdue Cytometry Mailing List: RE: DNA analysis
software ... J. Herbert Technical Support Manager Verity Software
House ... http://flowcyt.cyto.purdue.edu/hmarchiv/... -
1 week ago
Cytometry Mailing List: Re: DNA analysis software ... vsh.com] > Sent:
Wednesday, February 21, 2007 10:30 AM > To: Cytometry Mailing List >
Subject: RE: DNA ... J. Herbert > Technical Support Manager > Verity
Software House ... http://www.cyto.purdue.edu/hmarchiv/Curr...
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1 week ago
Purdue Cytometry Mailing List: DNA analysis software
> ... a conversion at no charge. I invite you to contact Verity
> Software
> House directly with any specific questions or issues. Best
> regards ...
> issues. Best regards, Don Donald J. Herbert Technical Support Manager
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1 week ago
On Aug 20, 2004, at 2:25 PM, J. Paul Robinson wrote:
> > > ummm....Mario says..
> > > "In life sciences -- particularly in research life sciences --
> > > probably 50 to 70% of research laboratories used
> > > Macs"....while I have a passionate dislike for Windows......is
> > > this really true ??? or is the key word there "used"?? (Ok...I
> > > have put on my helmet and armor....waiting...)
> > > paul
> > >> For all of the mr groupies out there in cytometry cyberspace. Don't
> > >> wet your
> > >> pocket protectors over this.
> > >> Honestly though, well deserved praise for Mario & the Tree Star group:
> > >>http://www.apple.com/science/profiles/ro...
1 week ago
Purdue Cytometry Mailing List: Confirm Your Bowdoin Course Regis
You may email us at s <BLOCKED::mailto:[email protected]> [email protected] to
confirm your status. Best regards, Verity Software House 45A Augusta
Road Topsham, ...
flowcyt.cyto.purdue.edu/hmarchiv/2006/... - 6k - Cached - Similar
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1 week ago
To subscribe to the image cytometry mailing list, mail a subscription
request .... LABCAT Software Home Page has on- line sales and support
for a line of PC ...
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1 week ago
Purdue Cytometry Mailing List: ModFit LT 3.1 Service Pack 1 now ...
For a complete description, see CYTOMETRY 53A:2, 103-111 (June,
2003). ... Best regards, Mark Mark E. Munson Sales Manager Verity
Software House, ...
Purdue Cytometry Mailing List: ModFit LT 3.1 Service Pack 1 now available for download... - 6k - Cached - Similar
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1 week ago
Why were all these links removed from Verity Soft Ware House ...
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1 week ago
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... http://www.wehi.edu.au/cytometry/WEASELv... > > Winlist 3d
from
Verity House Software (Verity Software House) ... Yes, I live off FlowJo sales,
and that's a blatantly ...
http://www.cyto.purdue.edu/hmarchiv/2006... - 8.5KB
71%
1 week ago
Cytometry Software
Purdue University Cytometry Laboratories. Catalog of Free Flow
Cytometry Software ... WinMDI Software - Joe Trotter. FCS Assistant -
Ray Hicks. FCS Press - Ray Hicks ...
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1 week ago
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13 Sep 07
1 week ago
From: Adam Treister ([email protected])
Date: Mon Apr 22 2002 - 00:15:07 EST
* Next message: b cotleur: "2-me in culture media:summary"
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______________________________________...
Only two more weeks until ISAC, that biennial bacchanalia of flower
power
and fun! So I hope you'll excuse a bunch of blatantly commercial
announcements to the list, but endulge me this one time and read on.

At the show we'll be releasing FlowJo Version 4. We've got new
platforms
for overlaying and clustering, we've made it work better across the
Internet, added all sorts of new
1 week ago
For those who haven't found it yet, we've unveiled a spanking new
FlowJo
website. Flowjo.com is chuck full of new content, functionality and
spunk.
Automated price quotes, online ordering, a FAQ that will guide you to
new
depths of understanding, and none of that awful yellow on black text.
The
search engine even works. No ads & cookie-free. Check it out.


Specifically, you should check our pricing. Prices are going up on
May 10.
It has been a number of years since we've changed our prices and with
the
development of the OSX and PC versions, it¹s time for a leap. I
guess
there's no such thing as a free launch. Anyway, this may be a great
time to
buy that FlowJo ten pack you've been thinking about. Licenses
purchased
before May 10 are entitled to a year of free upgrades, including the
4.0
release.


Unleash the flower power!


Adam
--
Adam Treister
Tree Star,
1 week ago
Fluorescence Compensation
Jennifer Wilshire, PhD [email protected] MetroFlow Meeting,
Princeton, NJ .... Purdue Cytometry Mailing list - http://www.cyto.purdue.edu.
Practical Flow ...
www.metroflow.org/archive/2004Jan06/ab... -
39k - Cached - Similar pages - Note this
1 week ago
Purdue Cytometry Mailing List: Re: Nonreadable FCS 3 files from
From : J. Paul Robinson <[email protected]> ... For data
analysis we want to > use a centraly designed templates made in Flow
Jo software. ...
Purdue Cytometry Mailing List: Re: Nonreadable FCS 3 files from... - 8k - Cached - Similar
pages - Note this
1 week ago
Useful Links & Resources - Flow Cytometry Site
General. Purdue University Cytometry Labs (J. Paul Robinson) ...
Software. FlowJo. FlowJo Home FACSPress and FACSAssistant.
http://www.fcspress.com/ ...
idi.harvard.edu/content/facilities/cyt...
- 42k - Cached - Similar pages - Note this


Meeting 2007
J Paul Robinson (Purdue University, USA). Multicolour Flow
Cytometry ... Cytek Development, Distrilab, FlowJo, Guava
Technologies, Invitrogen, ...
Meeting 2007... - 8k - Cached - Similar
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1 week ago
Flocyte course in Mexico City, march 3 to 7th 2008
This message: [ Message body ] [ More options ]
Related messages: [ Next message ] [ Previous message ]
From: Candela Iglesias Chiesa <[email protected]>
Date: Thu Jan 03 2008 - 11:39:14 EST

Para mensaje en espaqol, ver mas abajo. For message in Spanish, see
below.


Dear flowers,


The Flow Cytometry Core Facility at the Center for Research in
Infectious
Diseases (CIENI), at the Instituto Nacional de Enfermedades
Respiratorias in
Mexico City is pleased to host the first FloCyte Regional Training
Program
in Mexico! We are offering a full week of Flow Cytometry courses in
March
2008. Dorothy Lewis and Carol Oxford will be our instructors for the
5 days
of courses. You can register for each individually or receive a
special
discount, and register for all five days for $200 off the regular
price!
Please note that the courses will be held in English, but we'll have
translate
1 week ago
The Basic Flow Cytometry course will be held March 3-4, 2008. This
session
is excellent for beginners, and a great course for intermediate-
advanced
users looking for a stronger foundation in flow cytometry basic
theory.
Link to the course curriculum:
<>http://www.flocyte.com/FRTP/basic_curric...
http://www.flocyte.com/FRTP/basic_curric...


The Multiparameter Flow Cytometry and Compensation course will be
held
March
5, 2008. This advanced level course is for flow cytometry users who
are
interested in continuing their education in the field, and who plan
to
do
sophisticated polychromatic flow cytometry experimental analyses. It
is
suggested that participates have a good foundation in basic flow
theory and
operation prior to attending this course, or have attended the basic
course.
Link to course curriculum:
<>http://www.flocyte.com/FRTP/multiparamet...
http://www.flocyte.com/FRTP/multiparamet...
1 week ago
Please invite others you know who would also benefit who may not be
on
the
Purdue List Server.
1 week ago
Visit the website for more information on the FloCyte Associates
Regional
Training Program. <>http://www.flocyte.com/FRTP/FRTPHome.htm...
FloCyte Regional Training Program
Also, as the hostess of this event, please feel free to contact me
for
more
details.


Hope to see you there!


Marma Candela Iglesias, PhD


Centro de Investigacion en Enfermedades Infecciosas (CIENI)


Instituto Nacional de Enfermedades Respiratorias


Calzada de Tlalpan 4502. Col. Seccion XVI


CP 14080. Deleg. Tlalpan


Mixico DF.


tel y fax +52 (55) 56 66 79 85


[email protected]


[email protected]
1 week ago
Pueden encontrar mas informacisn sobre el Programa Regional de
Entrenamiento
de la Asociacisn Flocyte en FloCyte Regional Training Program


Asimismo, como la anfitriona de este evento, estoy a su disposicisn
si
requieren mas detalles.


!Espero verlos en el curso!


Saludos cordiales,


Marma Candela Iglesias, PhD


Centro de Investigacion en Enfermedades Infecciosas (CIENI)


Instituto Nacional de Enfermedades Respiratorias


Calzada de Tlalpan 4502. Col. Seccion XVI


CP 14080. Deleg. Tlalpan


Mixico DF.
1 week ago
Received on Thu Jan 3 18:38:00 2008
This message: [ Message body ]
Next message: Susan DeMaggio: "Budapest on HDTV - a little off the
flow topic, but relevant"
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PBMCs
results in sticky cells - The replies"
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This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 -
03:12:00
1 week ago
Only two more weeks until ISAC, that biennial bacchanalia of flower
power and fun! So I hope you'll excuse a bunch of blatantly commercial announcements to the list, but endulge me this one time and read on.
At the show we'll be releasing FlowJo Version 4. We've got new
platforms for overlaying and clustering, we've made it work better across the Internet, added all sorts of new conveniences, and spiffed it up with the new OS X look and feel. What was already the best analysis software in flow cytometry has gotten a whole lot better. At the meeting, Tree Star is proud to be sponsoring the CyberCafe, your link to home and responsibility while you're in carefree San Diego. Check your email, surf the web, download the slides you'll be presenting at ten. A cadre of California companies has ontributed to bring in a premier local roaster to satisfy all your latte urges. We hope you'll all drop by and see what we mean when we say we're committed to Java.
1 week ago
The network is
going
wireless this year. Just pop a 802.11 card in your laptop, and while
your
neighbor plays solitaire through the keynote, you can be reading e-
mail.


We're going to open the CyberCafe with the Second Biennial FlowJo
Users
Group Meeting,. Saturday night May 4 at 8PM. The first user group
meeting
was cancelled for lack of interest when Dave Novo brought in a case
and a
half of French wine, so this year we're going to try real hard to
assemble
to the point where we can see the show of hands on something before we
disband in search of alcohol. We'll have a whole bunch of Macs
running
FlowJo v4 under OS X, and you can bring your own data and get into big
arguments about compensation. Be the first to get the newest FlowJo
t-shirt.
All you Windows fans out the**re, come by our booth to see FlowJo
running on a PC! We'll be previewing the long awaited Java version of FlowJo. We're still not ready to release it,
1 week ago
but we'll be giving a peak as to what
it is going to do.
For those who haven't found it yet, we've unveiled a spanking new
FlowJo website. Flowjo.com is chuck full of new content, functionality and
spunk.
Automated price quotes, online ordering, a FAQ that will guide you to
new depths of understanding, and none of that awful yellow on black text.
The search engine even works. No ads & cookie-free. Check it out.
Specifically, you should check our pricing. Prices are going up on
May 10.
It has been a number of years since we've changed our prices and with
the development of the OSX and PC versions, it¹s time for a leap. I
guess there's no such thing as a free launch. Anyway, this may be a great
time to buy that FlowJo ten pack you've been thinking about. Licenses
purchased before May 10 are entitled to a year of free upgrades, including the 4.0 release.Unleash the flower power!
Adam Adam Treister
Tree Star, Inc.
ph: 800-366-6045
1 week ago
fax: 1-650-508-9186
[email protected] Tree Star Inc., Flowjo, flow cytometry, analyze flow cytometry data, flow cytometry analysis, cytometry,
--------------------------------------...
______________________________________...
1 week ago
From: Novo, David <[email protected]>
Date: Fri Aug 17 2007 - 18:52:53 EDT


Hello Kate,


The FC 500 data files are indeed a complicated beast that give you a
lot
of information. There are indeed two separate data sets inside the one
FC500 file. This is allowed by the FCS standard.


The first data set is a FCS 2.0 file. This is quite a typical FCS 2.0
file, stored in 10 bit with the data already saved compensated and
converted to log (if necessary). This is quite clever to put it there
because any analysis software that can read FCS 2.0 files should be
able
to read this file, and not even know that there was a second data set
hiding afterwards. I am not sure why the BD CBA software would not be
able to handle this, maybe there is a hidden setting or something that
allows this.
1 week ago
The second data set is a high resolution data set that is in FCS 3.0
format. This contains all the high resolution linear data, that is
stored uncompensated, and is ideal for performing software
compensation.
I guess our web site is either too high, or too low, and fell out of
the
range of your previous search :) FCS Express can read both the FCS
2.0
and FCS 3.0 portion of the FCS 500 data files. You can even export
them
as individual files if you like. When loading the files you can either
default to load the FCS 2.0 or FCS 3.0 portion, or be prompted which
to
choose.

I also know that WinList can read both portions of the FC500 file. I
am
not familiar with FlowJo's capabilities in this regard. If you contact
them, I am sure they will be glad to answer this point.


-Dave


-----------------------------------


David Novo


President


De Novo Software


[email protected]
1 week ago
From: J. Paul Robinson <[email protected]>
Date: Mon Jun 04 2007 - 23:40:22 EDT
Dear Colleagues
We are almost finished one of the most exciting projects we have
done.
It is our new DVD "Cytometry - 60 years of Innovation". It's our
first DVD. Actually, it's a two DVD set - one DVD works on your DVD player
and
the other on your computer. The contents are really fantastic -
original
video of Mack Fulwyler explaining how he invented the cell sorter,
including pages from his original lab notebook on how he came up with
the ideas...Len Herzenberg showing how he developed the technology
into
something that impacted the whole world. Howard Shapiro - one of us
who
has contributed his life to the field, as well as bringing the key
technology comes to you in song! Dozens of the leaders of the field
tell
us how they see cytometry in the past, present and future. Of course,
there are lots of educational features on the disc as well -
lectures,
1 week ago
powerpoint files galore, and a plethora of educational materials to
enhance your role as a student, staff member, teacher or core
manager.


The way we bring these educational discs free to you all is via our
sponsors. Sony has graciously donated the production from the Indiana
plant - you probably didn't know that your downloaded songs, dvds and
cds are probably mostly produced in good ole' Indiana - but they
are!!
1 week ago
This 60 year celebration of cytometry - brings Diamond Sponsors Bangs
Labs and Beckman-Coulter who lead our sponsors. Both companies bring
a
depth of knowledge and skills to cytometry. Beckman-Coulter started
the
field with the invention by Wallace Coulter of the Coulter counter
over
50 years ago. Bangs Labs has been a leader in bead technology for
many
years. Both companies have been two of our strongest supporters over
the
many years of doing these educational projects. Thank you for that
support.
1 week ago
Major sponsors who generously supported this initiative include
Partec,
Invitrogen, Dako, Guava and John Wiley & Sons, publisher of Cytometry
Part A. These major players in the field of cytometry are known to
all
of you.


We also acknowledge support from Hamamatsu, Southern Biotech,
Compucyte,
Phoenix Flow systems, Spherotech, Accuri Cytometers, EXBIO
Praha,A.S.,
Duke Scientific, Coherent, Q-Imaging and Verity Software. All of
these
companies provide quality cytometry related resources and we are very
grateful for their support of this really exciting educational and
historic project.
1 week ago
Finally, I want to give one last opportunity for any of you to submit
materials that might enhance this project. We have a few days to go
before the project is literally "etched" in plastic! Send your
materials
to Monica Shively, the producer of this project.
([email protected] ). If you want to see a sneak preview
of
the project, go to http://www.cyto.purdue.edu/flowcyt/cdser...


We will be distributing 10,000 copies of this two DVD boxed set - I
hope
you all get a chance to get a copy. Please ask your sales rep from
one
of the above companies they will all have copies for distribution.
1 week ago
They
are the best source of this historic DVD set. Purdue University
Cytometry Laboratories, who also bring you this discussion board are
pleased to continue bringing innovative projects on cytometry to you.
I
hope you enjoy this one. It's the best we have ever done!


Kind regards


J. Paul Robinson
Director, Purdue University Cytometry Laboratories
1 week ago
--
J. Paul Robinson
SVM Professor of Cytomics
Professor of Immunopharmacology & Biomedical Engineering
Director, Purdue University Cytometry Laboratories
President, International Society for Analytical Cytology


Purdue University Cytometry Laboratories
Bindley Bioscience Center
1203 West State Street
Discovery Park, Purdue University
West Lafayette, IN 47907-2057
Ph (765) 494 0757; Fax (765) 494 0517
email: [email protected]
Flow Cytometry, cytometry,Confocal Microscopy, multiphoton microscopy, Image Analysis, clinical cytometry, cytometry, high content screening, imaging, Visualization, Cytometry email discussion, Cytometry CD-ROM, Purdue University Cytometry Laboratori


--
J. Paul Robinson
SVM Professor of Cytomics
Professor of Immunopharmacology & Biomedical Engineering
Director, Purdue University Cytometry Laboratories
President, International Society for Analytical Cytology


Purdue University Cytometry Laboratories
Bindley Bioscience Center
1203 West State Street
Discovery Park, Purdue University
West Lafayette, IN 47907-2057
Ph (765) 494 0757; Fax (765) 494 0517
email: [email protected]
Flow Cytometry, cytometry,Confocal Microscopy, multiphoton microscopy, Image Analysis, clinical cytometry, cytometry, high content screening, imaging, Visualization, Cytometry email discussion, Cytometry CD-ROM, Purdue University Cytometry Laboratori
1 week ago
Join ISAC - Welcome to the ISAC! - Mambo


Change lives today - Cytometry for Life
1 week ago
Purdue Cytometry Mailing List: RE: CFSE graphs (UNCLASSIFIED)
... Verity's MODFIT software has a very nice tool for ... The
information contained in this electronic e-mail transmission and any attachments are intended only for the ... http://flowcyt.cyto.purdue.edu/hmarchiv/... - 9.3KB

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1 week ago
Purdue Cytometry Mailing List: Thanks for the suggestions - was
... Purdue Cytometry Mailing List: Thanks for the suggestions -
was ... cytometry/WEASELv2.html Winlist 3d from Verity House Software (Verity Software House) ...
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1 week ago
* Software recommendations for offline analysis in PC-land
o VSH - Tech Support (Mon Oct 25 2004 - 13:53:09 EST)
o Cytometry (Tue Oct 19 2004 - 23:42:40 EST)
o Zand, Martin (Mon Oct 18 2004 - 15:05:30 EST)
RE: Software recommendations for offline analysis in PC-land
* This message: [ Message body ] [ More options ]
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reply to ]
From: VSH - Tech Support <[email protected]>
Date: Mon Oct 25 2004 - 13:53:09 EST
Hello, Dr. Zand,

As a follow-up to your posting on the cytometry mailing list, I should
explain that only the first copy of WinList is $2000 (the "primary
license"), and after that all "additional user licenses" are only $400
each,
for the PC or for the Mac. We also offer a software "battery" option
that
allows you to use WinList for as little as $250.
Please contact me directly to discuss these options in more detail.

Best regards,
Mark

Mark E. Munson
Sales Manager
Verity Software
1 week ago
Purdue Cytometry Mailing List: Re: HP to Windows NT reader
... Rich: ... I have been very happy with Verity's WHPPC package. It
consists of an interface card on the ... It consists of an interface card on the PC and software for both the PC and HP. It is very fast (about 10X ...
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Purdue Cytometry Mailing List: Re: FACS simulator?
... Verity did a good job, but with a different emphasis, on their
software ... Verity did a good job, but with a different emphasis, on their software here http://www.vsh.com/downloads/Simulator/C... Hope this helps Geoff ...
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1 week ago
Re: SPAM-LOW: Re: Manual vs. Auto compensation
* This message: [ Message body ] [ More options ]
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to ] [ Next in thread ]
From: FloCyte Associates, INC <[email protected]>
Date: Wed Feb 13 2008 - 19:41:27 EST
Hi Mario
I have to commend YOU too! Two of the good explanation were from
FloCyte graduates - who learned from YOUR slides!!
Sue
1 week ago
At 11:54 AM 2/12/2008, you wrote:
Let me add one comment to this... Maciej is right that minor
miscompensation will not affect your results if you are gating on
easily-separable populations. It can strongly affect your results if
you are gating on difficult-to-separate populations ("smears").

Most importantly, it will INVALIDATE your results if you are relying
on MFI (fluorescence intensity) calculations. For those who are doing
MFI analyses, it is absolutely essential to validate your compensation
settings!

mr

(PS, it is really encouraging that I saw so many excellent
explanations of compensation on the mailing list recently! Really
outstanding.)
1 week ago
--- Maciej Simm <[email protected]> wrote:

Colleagues,

It's very difficult to answer the question "should I
do it by hand or
computer" without the whole context of the specific
scenario when one
fails and the other works.

Instead, we can focus on a broader question "is my
compensationcorrect?"
1 week ago
Mathematically, we can estimate validity of our
compensation matrices
by comparing medians - for example, the median of
the positives is
equal to the median of the negatives. Medians are
better than mean/
GM's because they aren't affected by outliers and
better represent
central tendency of your data.
1 week ago
Since biexponential transformation (aka logicle
display, etc..) became
popular in many software packages, there is an
easier way to 'eyeball'
bad compensation - enable this scaling and see if
your single-
negatives (some call them single-positives, I'm a
sheath tank half-
empty kinda guy ;) ) are symmetrically distributed
around the X=0 /
Y=0 lines similar to your double-negatives. If not,
there's either
over comp, or undercomp. Here's an example, with
hypothetical X/Y
parameter distributions:
1 week ago
http://cd4cd8.com/1.png proper comp -1 % - the X+/Y-
population leans
upward, suggesting undercompensation.
http://cd4cd8.com/2.png proper comp +1% - the X+/Y-
population is
sagging toward X axis. Over comp'd.
http://cd4cd8.com/3.png proper comp (correct values)
- the centers of
X-/Y- and X+/Y- are aligned.

hope this helps,
1 week ago
Maciej Simm
TreeStar Inc.






PS. I vote for pistachio ice cream.




I have people in the lab who complain about me
not doing manual
compensations and instead have the Aria calculate
them for me. And
when I have the Aria compensate, they want to
manually compensate
after they record the data because the plots
"don't look right." I
mean, isn't that simply producing "make-belief"
data? Or am I
missing something?
1 week ago
----Original Message-----
From: FloCyte Associates, INC [[email protected]]
Sent: Thu 3/20/2008 2:16 PM
To: cyto-inbox
Subject: RE: Bay bioscience opens US office and introduces its JSAN
cell sorting and analysis system there

No this is not acceptable! I'm sorry, I just don't get it! I agree
100% with Roland.

I can't understand your position at all? WHO pays the majority of
the expenses for meetings? Vendors! Without them your cost to attend
ISAC would probably triple or quadruple!! Who allows you to have
very cheap or FREE local users' group meetings? Vendors! Who solves
issues with vendor services?? VENDORS! Why on earth would you
exclude vendors from the discussion???
1 week ago
And actually SOME people welcome messages from vendors... Vendors can
solve a lot of your problems! The Boston Area high speed sorter
list, for example, welcomes vendors responses and they get
results. The vendors HEAR their requests and respond! I've learned
a LOT from vendors! Without messages from Vendors, how do you find
out about new products and services? How often would you go to the
ISAC website to look for a new product you don't even know
exists? Or how do you know there is some training opportunity
happening in your area? OR how do Vendors know what you need and how
do you get your service / technical questions heard by all vendors?
1 week ago
Shutting them out isn't the answer to your mail problems! Roland's
answer was eloquent! And, although we've discussed this often,
nothing has ever evolved that is a better suggestion! If you don't
like messages from vendors, just filter them out, and having
[Company:] in the subject time is a perfect way to do that!

I'm sorry, I just don't get it!! Why would you shoot yourself in
the foot? Vendors - you can put my email address on your mailing
list!! Just put [Company:] in the subject line! I'll filter them
and decide what I want to read or not read later! Just like I filter
this list!
Sue
1 week ago
Received on Mon Mar 24 17:18:00 2008
* This message: [ Message body ]
* Next message: Ray Hicks: "FL-6 issues - a not-so-nonsensical
label!!"
* Previous message: Adrian Smith: "Re: Bay bioscience opens US office
and introduces its JSAN cell sorting and analysis system there"
* In reply to: FloCyte Associates, INC: "RE: Bay bioscience opens US
office and introduces its JSAN cell sorting and analysis system
there"
* Next in thread: [email protected]: "RE: Bay bioscience opens US
office and introduces its JSAN cell sorting and analysis system
there"
* Reply: [email protected]: "RE: Bay bioscience opens US office and
introduces its JSAN cell sorting and analysis system there"
* Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
Subject ] [ By Author ] [ By messages with attachments ]
This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 -
03:12:00 EST
1 week ago
From: <[email protected]: Fri Mar 21 2008 - 19:47:53 EDT
Dear Friends and Colleagues,
Without belaboring the point, while I find this an interesting
suggestion,
it would imply that I should place [Company] in the subject line
because I
work for a company. All rules have their excess, and I would submit
that J
Paul (actually Steve) and company have done a great job maintaining
the
integrity of this list. If an occasional message is allowed thru that
has
potential commercial implications, I would rather have that than
complete
(or no) censorship. If individuals wish to express their displeasure
at a
specific posting (irrespective of the source) this allows discussion,
and
if necessary, re-calibration of the gating algorithm. And regarding
vendors monitoring the list versus actively soliciting messages, I
suspect
that most monitor this list to gather useful information, much of
potential use to their customers. But this is quite different from
"open
access" for solicitation.
1 week ago
While I'm here, some additional comments:
1.) Cost of the ISAC meeting. I find the registration of $650 (US) a
bit
steep for a meeting. While I was on ISAC Council, a resolution was
passed
by Council that the Congress be shortened (in an attempt to reduce
the
registration fee and overall cost) and be held annually. The
combination
of a $650 registration fee plus hotel costs (esp in Europe- for US
scientists) makes it difficult for many labs to bring key technicians
and
post-docs; it even makes it difficult for many senior scientists to
justify. To put this in a bit of perspective, the American Asc for
Cancer
Research charges a $425 (US) registration fee to their members for
their 4
day. An important difference is that the AACR has SIGNIFICANT
industrial
support. We need to do as much as possible to make our meeting's
overall
cost "reasonable". ISAC must do everything it can to keep the overall
meeting costs (registration, airfare, hotels and meals) to a minimum
(this
ain't ASCO).
1 week ago
2.) Vendor support. This is essential for ISAC or any other
professional
society. At one point, I was chair of the committee that raised
donations
for the ISAC Congress, and approached every company that I could think
of
that sold products to our membership quite shamelessly. Every society
does
this because it helps lower the cost of the meeting (or should) for
the
individual attendees. And by the way, the money the vendors use comes
from
the customers. Donations are thus a means to widen the available
attendees
who will buy their products. The approach must be balanced.
1 week ago
3) Meeting location. While I appreciate this is a problematic issue,
refer
back to item "1. At one point, ISAC restricted the Congress to
"smaller"
venues to keep us together. Much science gets discussed (in places
that
tend to serve alcohol) at ISAC, and that's one reason I attend
(discussion, not libations). Where will we gather in Budapest (after
poster sessions)? Unlikely to be the InterContinental Hotel - I
probably
could not afford one beer there.
4) What is the focus of ISAC? I'm running for Clinical Councilor
(sorry, I
too had to get in my pitch), so my potential constituency for the
most
part has a limited focus. But the society needs to have a balanced
focus
on flow and image cytometry. And I don't think that DNA, RNA or
protein
arrays should be our soup!. Our unit of measurement is the cell, and
once
you bust it up, someone with better technical expertise should be
telling
scientists and clinicians what best to do. That said, we should
communicate and work with "non-cellular
1 week ago
ientists where our
interests
and technologies intersect.
I need to go home for the weekend. A Happy Easter to all.
Sincerely,

Vince

p.s. I'm running for Clinical Councilor. If you live in Chicago vote
early
and often


T. Vincent Shankey, Ph.D.
Advanced Technology Center
Beckman Coulter, Inc.
[email protected]
(305)-380-2430
2 days ago
Purdue Cytometry Mailing List: Re: Nonreadable FCS 3 files from
From : J. Paul Robinson <[email protected]> ... For data
analysis we want to > use a centraly designed templates made in Flow
Jo software. ...
Purdue Cytometry Mailing List: Re: Nonreadable FCS 3 files from... - 8k - Cached - Similar
pages - Note this

1 day ago

Useful Links & Resources - Flow Cytometry Site
General. Purdue University Cytometry Labs (J. Paul Robinson) ...
Software. FlowJo. FlowJo Home FACSPress and FACSAssistant.
http://www.fcspress.com/ ...
idi.harvard.edu/content/facilities/cyt...
- 42k - Cached - Similar pages - Note this
2 days ago
FCSPress Training Sessions?
From: Ray Hicks ([email protected])
Date: Thu Oct 11 2001 - 19:42:36 EST
In reply to: Jennifer Wilshire: "FlowJo Training Sessions (Midwest)"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
--------------------------------------...
Most of you will find FCSPress so easy to use that you won't need
training
sessions. You can download a copy to try it out for yourselves at
http://www.fcspress.com/html/FCSPArea.ht...
where you'll also find documentation and details of a special offer.
If you find you DO need some assistance using FCSPress, I'm planning
on
staying in Cambridge for quite some time, and I'd be happy to answer
any of
your queries via e-mail at [email protected]
Cheers,
Ray
--
e-mail mailto:[email protected]
Web http://www.FCSPress.com
Tel +44 797 453 8647
+44 12
2 days ago
Purdue Cytometry Mailing List: RE: Diva 4.0 files ... Mark E.
Munson Sales Manager Verity Software House, Inc. 45A Augusta Road PO
Box 247 Topsham, ME 04086 Phone: 207-729-6767 x191 Fax:
207-729-5443 ...http://www.cyto.purdue.edu/hmarchiv/2004... 6.2KB
74%
2 days ago
FlowJo Training Sessions (Midwest)

From: Jennifer Wilshire ([email protected])
Date: Thu Oct 11 2001 - 06:20:11 EST

* Next message: Richard Grenfell: "Multiple plate loader?"
* Previous message: William King: "Searching for pig anti-CD34"
* Next in thread: Ray Hicks: "FCSPress Training Sessions?"
* Reply: Ray Hicks: "FCSPress Training Sessions?"
* Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]

I will be in Wisconsin, Minnesota, and Iowa offering FlowJo seminars and training sessions next week. All of the hosting sites have graciously opened the sessions to members of the Cytometry list. Times and locations are listed below. Thanks, Jennifer Wilshire FLOWJO SEMINARS ______________________________________ Monday, October 15th University Hospitals Clinical Sciences Center 600 Highland Ave, Madison WI Room K4/418 10:00 AM ______________________________________ Tuesday,
2 days ago
October 16th University of Minnesota Cancer Center 425 E. River Road Room 450 9:00 AM * Introduction to FlowJo 10:30 AM * Advanced Topics _______________________________________ Tuesday, October 16th University of Minnesota, St. Paul Building AS/VM Room 295M 2:00 PM _______________________________________ Friday, October 19th University of Iowa South East 310 General Hospital Bean Conference Room 10:00 AM _______________________________________ If you are interested in hosting a FlowJo seminar, please let me know. Upcoming trips are in the planning stages for: -NJ, PA, DC -Florida. -Boston (home) Jennifer Wilshire, Ph.D. Application Scientist [email protected] Tree Star, Inc. FlowJo Home
2 days ago
Recent FlowJo announcement
From: Steve Kelley ([email protected])
Date: Wed Dec 30 1998 - 06:13:04 EST

* Next message: Steve Kelley: "Possible minor disruption"
* Previous message: Mark A. Corio: "Chemdex no help..."
* Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]

I know that many people on the mailing list are adamantly opposed to anything that even looks a little commercial, and to

try to forestall any possible complaints about the FlowJo announcement, I'll explain what I'd like to see.

In my opinion, announcements about new products, and product updates are completely appropriate as long as they aren't abused

. I've never wanted to specifically encourage that, because I really don't want to be put into a position of having to decide whether a message is an "

announcement"

or an "

advertisement".
2 days ago
The companies involved in our cytometry community have always been extremely good 'citizens' as far as I can tell.

We have representatives of many companies on the list, and they have always had the power to make my life miserable, and the list no more than

junk mail.

Instead, they have helped build this mailing list into one of the most useful around, through their contributions and responses. oI'm not going to try to set specific rules about what people can say about their wn products, and when they can say it. I'll just ask that everyone continue to show restraint; that the commercial representatives ask themselves before they submit an announcement whether they'd mind seeing every other company in the business sending the same message they are about to, and that the

non- commercial (and anti-commercial) people accept discreet announcements as simple information,
2 days ago
and continue the sometimes brisk discussions about problems and benefits of particular products, alongside the purely scientific (and occasionally purely entertaining) conversation. Steve Steve Kelley

[email protected]

Purdue University

Cytometry Laboratories

(765) 494-0757

-- voice B050 Hansen LSRB,

Purdue University

(765) 494-0517

-- fax

West Lafayette, Indiana, 47907

-- End --

* Next message: Steve Kelley: "Possible minor disruption"
* Previous message: Mark A. Corio: "Chemdex no help..."
* Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:35:29 EST
2 days ago
Purdue Cytometry Mailing List: RE: Software for merging FSC 3 fi
... Purdue Cytometry Mailing List: RE: Software for merging FSC ...
J.
Herbert Technical Support Manager Verity Software House, Inc. PO Box
247 ...http://www.cyto.purdue.edu/hmarchiv/2005... 6.6KB
73%
2 days ago
Purdue Cytometry Mailing List: Announcing User Forum at
Verity ...
Verity Software House is pleased to announce the creation of a Verity
User's Forum on our ...
http://www.cyto.purdue.edu/hmarchiv/2002... - 4.6KB
78%
2 days ago
Purdue Cytometry Mailing List: Re: Log-like transforms
... Purdue Cytometry Mailing List: Re: Log-like transforms ...
purposes. > > > > Bruce > > > > C. Bruce Bagwell MD, Ph.D. > >
President > > Verity Software House > > 45A Augusta Road > > PO Box
247 > > Topsham, ME 04086 ...
http://www.cyto.purdue.edu/hmarchiv/2006... - 10.0KB
2 days ago
Purdue Cytometry Mailing List: RE: utility software to convert
fcs1.0 to fcs2.0 ... Since you are using the PC, I suggest you
contact
Verity Software (207-729-6767) . I quickly tried this function this
AM ...
http://www.cyto.purdue.edu/hmarchiv/1998... - 7.8KB
73%
 
On Dec 25 2007, 1:14 pm, Mitch Haynes <mitchhay...@gmail.com> wrote:
> On 11 Dec, 00:52, Mitch Haynes <mitchhay...@gmail.com> wrote:> Purdue University Mailing List Service Terms of Use
> > Purdue University provides the Mailing List Service for activities
> > and
> > associated administrative functions supporting its mission of
> > learning, discovery, and engagement. Use of the Mailing List Service
> > is subject to Purdue University policies, in particular the policies
> > described in "Use of Electronic Mail" and "IT Resource Acceptable Use
> > Policy".
> > Acceptable Use:
> > The Mailing List Service is to be used for University-related
> > educational, research and administrative purposes. Any use of the
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> > to the list. Persons subscribed to a Purdue University mailing list
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> > Consequences of Illegal or Improper Actions
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> > will be referred to the appropriate officials.
> > Actions that are in violation of the Terms of Use will result in the
> > blocking of posts from offending subscribers, removal of offending
> > subscribers from lists or the removal of lists.
> > The Mailing List Service administrators may monitor user activities
> > and access any of the service's files or information in the course of
> > performing normal system maintenance or while investigating policy
> > violations or improper use. Anyone using Purdue University mailing
> > list resources expressly consents to such monitoring and is advised
> > that if such monitoring reveals possible evidence of criminal
> > activity, Purdue will provide the evidence to law enforcement
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> > Additional Improper Actions
> > In addition to any actions prohibited by federal or state laws and
> > policies, or by Purdue University policies, the following specific
> > actions and uses of Purdue University Mailing List Facilities are
> > improper:
> > Concealing or misrepresenting names or affiliations in Purdue
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> > Using Purdue University mailing lists for organized political
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> > or political solicitation.
> > Sending any e-mail to a Purdue University mailing list that is not
> > consistent with the purpose of the list. Messages not relevant to the
> > purpose of the list will be viewed as unsolicited e-mail.
> > Continuing to send e-mail to a Purdue University mailing list after
> > the list owner has requested that the user stop sending to the list
> > because they are not following the guidelines or topic established
> > for
> > the list.
> > Harvesting e-mail addresses from another mailing list in order to
> > establish a Purdue University mailing list. It is permissible to post
> > a message to a closely related list to invite people to subscribe to
> > another Purdue University mailing list.
> > Harvesting e-mail addresses without proper authority from an
> > institution's directory, password file or any other source to
> > establish a Purdue University mailing list.
> > Harvesting e-mail addresses from a Purdue University mailing list for
> > use other than the intended purpose of the mailing list.
> > Subscribing any e-mail address to a Purdue University mailing list
> > except with proper authority or the individual's permission.
> > List Service Administration
> > Lists that are determined to not be active for extended periods will
> > be subject to removal. Warning will be given to list owners and list
> > members before such lists are discontinued.
> > The Mailing List Administrators reserve the right to trim archives
> > for
> > lists with archives that grow too large. A message will be sent to
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> > This Terms of Use statement is subject to modification. While a
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> > of Use statement.
> > ***************************************************************************************TALK
> > ABOUT PRESIDENT Geroge W. Bush and John F Kerry **YOUR POLITICAL
> > CHAT LINE**FLOW JO MARKETING.*******MARIO ROEDERER
> > mac vs. pc
> > * This message: [ Message body ] [ More options ]
> > * Related messages: [ Next message ] [ Previous message ] [ In
> > reply
> > to ] [ Next in thread ] [ Replies ]
> > From: Mario Roederer <roede...@drmr.com>
> > Date: Wed Aug 25 2004 - 15:10:25 EST
> > I have only one comment to make on this debate:
> > George W. Bush ("nuculer") uses a PC.
> > John F. Kerry uses a Mac (<Mac News First. | MacMinute News
> > kerry>).
> > Hmm.
> > mr
> > Received on Thu Aug 26 13:18:00 2004
> > * This message: [ Message body ]
> > * Next message: Bjvrnsson Sven G: "Cell cycle analysis with
> > DRAQ5"
> > * Previous message: WThrond...@genencor.com: "Re: dr. mr. on
> > Apple"
> > * In reply to: Robin Barclay: "Re: mr on Apple web site"
> > * Next in thread: Hirschkorn, Dale: "RE: mac vs. pc"
> > * Maybe reply: Hirschkorn, Dale: "RE: mac vs. pc"
> > * Maybe reply: Konz, Ric
> > hard: "RE: mac vs. pc"
> > * Reply: Joanne Lannigan: "RE: mac vs. pc"
> > * Reply: Robert C. Leif: "RE: mac vs. pc"
> > * Reply: Kristie Gordon: "Re: mac vs. pc"
> > * Maybe reply: Vandevert, Chris: "RE: mac vs. pc"
> > * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
> > Subject ] [ By Author ] [ By messages with attachments ]
> > This archive was generated by hypermail 2.1.8 : Tue Aug 31 2004 -
> > 03:12:05 EST
> > ****************************************************************************************COMMERCIAL
> > MARKETING THROUGH LIST BLATENT  VERITY HOUSE SOFTWARE
> > Analysis Software
> > This message: [ Message body ] [ More options ]
> > Related messages: [ Next message ] [ Previous message ]
> > From: VSH - Tech Support <t...@vsh.com>
> > Date: Wed Jun 30 2004 - 12:08:14 EST
> > David
> > At the risk of sounding commercial, WinList can do what you want. I
> > have
> > attached a few pictures showing the WinList Region Array feature.
> > With this
> > you can tell the program exactly how you want the regions created and
> > how
> > many. If you would like to try it for yourself you can download a
> > trial
> > version from our web sitewww.vsh.com.
> > Please let me know if you have any questions.
> > Best regards
> > Don
> > Donald J. Herbert
> > Technical Support Manager
> > Verity Software House, Inc.
> > PO Box 247
> > 45A Augusta Road
> > Topsham, ME, USA 04086
> > Phone: (207) 729-6767 ext.190
> > Fax: (207) 729-5443
> > email: t...@vsh.com
> > web:www.vsh.com
> > Verity Software House Has a Connected Search Engine attached selling
> > all software.

> > WHY DOES VERITY REQUEST ARTICLES TO BE DELETED AFTER READING?

> > WHY MAIL LIST IS DOWN THESE LINK ARE NOT ACTIVE

> > ****************************************************************************************
> > Purdue Cytometry Mailing List: Re: DNA analysis software
> > ... J. Herbert > Technical Support Manager > Verity Software
> > House ...
> > sender immediately by return e-mail, delete this e-mail and destroy
> > any copies. Any ...http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0755.htm-8.6KB 70%
> > ||||||||||||||||||||
> > 26 May 07
> > Find Similar
> > Highlight
> > ---------------------------------------------------------------------------
> > ¬-----
> > Purdue Cytometry Mailing List: DNA analysis software
> > ... a conversion at no charge. I invite you to contact Verity
> > Software
> > House directly with any specific questions or issues. Best
> > regards ...
> > issues. Best regards, Don Donald J. Herbert Technical Support Manager
> > Verity Software House ...http://www.cyto.purdue.edu/hmarchiv/Current/0753.htm-5.6KB 69%
> > ||||||||||||||||||||
> > 26 May 07
> > Find Similar
> > Highlight
> > ---------------------------------------------------------------------------
> > ¬-----
> > Purdue Cytometry Mailing List: Re: DNA analysis software
> > ... J. Herbert > Technical Support Manager > Verity Software
> > House ...
> > sender immediately by return e-mail, delete this e-mail and destroy
> > any copies. Any ...http://www.cyto.purdue.edu/hmarchiv/Current/0755.htm-8.6KB 69%
> > ||||||||||||||||||||
> > 26 May 07
> > Find Similar
> > Highlight
> > ---------------------------------------------------------------------------
> > ¬-----
> > Purdue Cytometry Mailing List: RE: DNA analysis software
> > ... Purdue Cytometry Mailing List: RE: DNA analysis software ... J.
> > Herbert Technical Support Manager Verity Software House ...http://www.cyto.purdue.edu/hmarchiv/Current/0747.htm-7.7KB 69%
> > ||||||||||||||||||||
> > 26 May 07
> > Find Similar
> > Highlight
> > ---------------------------------------------------------------------------
> > ¬-----
> > Purdue Cytometry Mailing List: RE: DNA analysis software
> > ... Purdue Cytometry Mailing List: RE: DNA analysis software ... J.
> > Herbert Technical Support Manager Verity Software House ...http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0747.htm-7.7KB 68%
> > ||||||||||||||||||||
> > 26 May 07
> > Find Similar
> > Highlight
> > ---------------------------------------------------------------------------
> > ¬-----
> > Purdue Cytometry Mailing List: RE: FL2H in PI cell cycle analysis
> > ... From: Verity Software House <ver...@vsh.com> Date: Fri Sep 07
> > 2007
> > - 17:30:59 EDT ... Verity Software House ...http://flowcyt.cyto.purdue.edu/hmarchiv/Current/1351.htm-7.2KB 66%
> > ||||||||||||||||||||
> > 12 Sep 07
> > Find Similar
> > Highlight
> > ANTI Commercial posting **NOTICE HOW THEY ADD A SMILEY
> > FACE**********FCS PRESS RAY HICKS
> > ****************************************************************************************
> > anti) Commercial Posting:)
> > This message: [ Message body ] [ More options ]
> > Related messages: [ Next message ] [ Previous message ]
> > From: Ray Hicks <rh...@cam.ac.uk>
> > Date: Wed Oct 13 2004 - 08:43:13 EST
> > Just a quick nopte to let people know that FCSPress is now shareware
> > (no time restrictions or feature reductions for free use, though if
> > you feel like like paying for it you can using paypal). It also now
> > works with Diva export files and runs ok under classic on OS 10.2 and
> > 10.3. I've discontinued FCS Assistant, which has fallen behind
> > FCSPress remarkably, and which failed to run as shareware on some
> > systems (it insisted you buy it or refused to run - FCSPress doesn't
> > even have a nag screen now - pesky things).
> > FCSPress is available fromwww.fcspress.com
> > Enjoy!
> > Ray
> > Received on Wed Oct 13 14:38:00 2004
> > This message: [ Message body ]
> > Next message: Mike: "Re: (almost) sterile sorting"
> > Previous message: Hans-Georg.Krey...@merck.de: "Once more: FACSstar
> > plus dual laser to give away (incl. location of the instrument)"
> > Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
> > Subject ]
> > [ By Author ] [ By messages with attachments ]
> > This archive was generated by hypermail 2.1.8 : Thu Oct 14 2004 -
> > 03:12:04 EST
> > Mr. Apple WEB IS Mario OWNER OF FLOW JO **MORE MARKETING
> > ****************************************************************************************
> > Re: mr on Apple web site
> > * This message: [ Message body ] [ More options ]
> > * Related messages: [ Next message ] [ Previous message ] [ In
> > reply
> > to ] [ Next in thread ] [ Replies ]
> > From: Robin Barclay <Robin.Barc...@ed.ac.uk>
> > Date: Wed Aug 25 2004 - 04:17:52 EST
> > Well .... some of us PC afficianados hate Macs just as much -
> > personally I
> > have hated them since they first made it difficult to access their
> > DOS
> > and
> > write programs in any language (especially for accessing/controlling
> > lab
> > apparatus) - back in the '70's when there were several different
> > options -
> > not just PC's. I am glad that the "PC" became a standard (there were
> > too
> > many diferent systems) and that IBM did not hang on to it the way
> > Apple kept
> > the Mac in house (you get more for your money with a PC because many
> > different people make them). You don't often get PC people knocking
> > Macs
> > the way that the Mac people knock PCs - there seems to be a lot of
> > Microsoft
> > paranoia. In my opinion PCs are much more common and versatile in
> > labs than
> > Macs - especially outside the USA - and will eventually become the
> > standard
> > for interfacing with lab equipment..... and they can "look cool" if
> > you shop
> > around for the right case if that's important to you.
> > Robin Barclay
> > Beverly Barton **STEERING COMMITY FOR* SCIENCE ADVISORY BOARD*****
> > Kanecki Associates Inc Discussion of FCS QUICK FACS 11 DELETED OFF THE
> > SITE!
> > MORE FLOW JO
> > ****************************************************************************************----- Original Message -----
> > From: "Beverly Barton" <barto...@UMDNJ.EDU>
> > To: cyto-inbox
> > Sent: Monday, August 23, 2004 10:09 PM
> > Subject: Re: mr on Apple web site
> > > Paul:
> > > I use Macs exclusively. Between home and labs, I have 7.
> > > I hate Windows (I tell everyone, I don't do Windows).
> > > Macs are what Windows wishes it could do. OS X is rock-solid,
> > > especially Jaguar (OS 10.3).
> > > BD guys-get with it and release CellQuest Pro for OS X!!!!!!!!!!!!!
> > > Oh, and Macs are way more photogenic. They look especially cool next
> > > to your flow cytometer. I just bought a new dual-boot G4 with
> > > flat-panel display for my flow.
> > > Beverly
> > > Beverly E. Barton, Ph.D.
> > > Assistant Professor
> > > Department of Surgery/Division of Urology
> > > UMDNJ-NJMS MSB G519
> > > 185 S. Orange Avenue
> > > Newark, New Jersey 07103
> > > Telephone 973-972-0662
> > > E-mail barto...@umdnj.edu
> > > Telefacsimile 973-972-3892
> > > On Aug 20, 2004, at 2:25 PM, J. Paul Robinson wrote:
> > > > ummm....Mario says..
> > > > "In life sciences -- particularly in research life sciences --
> > > > probably 50 to 70% of research laboratories used
> > > > Macs"....while I have a passionate dislike for Windows......is
> > > > this really true ??? or is the key word there "used"?? (Ok...I
> > > > have put on my helmet and armor....waiting...)
> > > > paul
> > > >> For all of the mr groupies out there in cytometry cyberspace. Don't
> > > >> wet your
> > > >> pocket protectors over this.
> > > >> Honestly though, well deserved praise for Mario & the Tree Star group:
> > > >>Apple - Science - Profiles - Dr. Mario Roederer
> > > >>> _______________________
> > > >>> Calman Prussin
> > > >>> Laboratory of Allergic Diseases
> > > >>> NIAID/ National Institutes of Health
> > > >> The information in this e-mail and any of its attachments is
> > > >> confidential
> > > >> and may contain sensitive information. It should not be used by
> > > >> anyone who
> > > >> is not the original intended recipient. If you have received this
> > > >> e-mail in
> > > >> error please inform the sender and delete it from your mailbox or any
> > > >> other
> > > >> storage devices. The National Institute of Allergy and Infectious
> > > >> Diseases
> > > >> (NIAID) shall not accept liability for any statements that are the
> > > >> senders own
> > > >> and not expressly made on behalf of the NIAID by one of its
> > > >> representatives.
> > > > J.Paul Robinson, PhD PH:(765)4940757
> > > > Professor of Immunopharmacology
> > > > Professor of Biomedical Engineering
> > > > Purdue University FAX:(765)4940517
> > > > EMAIL:j...@flowcyt.cyto.purdue.edu
> > > > WEB:http://www.cyto.purdue.edu
> > > > Have you seen our new HCS webpage?
> > > >High Content Screening Website

> > Received on Wed Aug 25 13:58:00 2004
> > * This message: [ Message body ]
> > * Next message: WThrond...@genencor.com: "Re: dr. mr. on Apple"
> > * Previous message: facs_copy: "Re: Sorting issues"
> > * In reply to: Beverly Barton: "Re: mr on Apple web site"
> > * Next in thread: Mario Roederer: "mac vs. pc"
> > * Reply: Mario Roederer: "mac vs. pc"
> > * Reply: Howard Shapiro: "Re: mr on Apple web site"
> > * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
> > Subject ] [ By Author ] [ By messages with attachments ]
> > This archive was generated by hypermail 2.1.8 : Fri Aug 27 2004 -
> > 03:12:05 EST

> > ***THE MENTION OF BD CYTOMETERS AND MORE MARKETING FOR FLOW JO **
> > MARIO

> > Randy T. Fischer**********
> > NIH/NIAMS

> > Mr. APPLE WEB SITE FLOW JO
> > ****************************************************************************************
> > RE: mr on Apple web site
> > * This message: [ Message body ] [ More options ]
> > * Related messages: [ Next message ] [ Previous message ]
> > [ Maybe in
> > reply to ]
> > From: Fischer, Randy (NIH/NIAMS) <fisch...@mail.nih.gov>
> > Date: Mon Aug 23 2004 - 16:18:55 EST
> > Paul,
> > I know that for a long time, anyone who used a BD flow cytometer had
> > ***************
> > little
> > choice but to use a MAC-data acquisition required it. And, one of
> > the
> > best
> > third party software packages was FlowJo which only ran on a MAC.
> > *******************
> > Mario,
> > being a FlowJock, naturally was heavily exposed to this atmosphere.
> > However, most of our other laboratory instrumentation appears to run
> > on the
> > IBM clone type of PC-ELISA reader, luminometer, ELISpot reader, and
> > AFFYMETRIX machine to name the first ones that come to mind. Of
> > course,
> > because Flow is such a key component and FlowJo works so well, we
> > have
> > MACs
> > (and associated cinema displays) at all of our desks. So, Mario is
> > probably
> > pretty close as I guess we now run about 50% MAC, and for most things
> > they
> > do run better than our PCs for similar applications, but mostly the
> > two
> > platforms tend to be used for very disparate applications and we need
> > both.
> > Wish more science companies would move to MAC, but as Microsoft
> > probably
> > owns a minority share in most companies, that will not likely happen
> > in our
> > lifetimes.
> > Haven't read the article yet, but hopefully Mario remembered to cite
> > you for
> > all the work on the Flow website/mailing list.
> > Randy T. Fischer
> > NIH/NIAMS
> > Building 10, Room 6D50
> > 9000 Rockville Pike
> > Bethesda, MD 20892
> > (301) 594-3537
> > fisch...@mail.nih.gov

> > Received on Tue Aug 24 16:18:00 2004
> > * This message: [ Message body ]
> > * Next message: Hans Jürgen Hoffmann: "SV: Any suggestion for
> > selection of ICAM-1 (CD54) antibody?"
> > * Previous message: J. Paul Robinson: "Re: mr on Apple web
> > site"
> > * Maybe in reply to: Prussin, Calman (NIH/NIAID): "mr on Apple
> > web
> > site"
> > * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
> > Subject ] [ By Author ] [ By messages with attachments ]
> > This archive was generated by hypermail 2.1.8 : Wed Aug 25 2004 -
> > 03:12:04 EST

> > PRISIDENT OF ISAC AND HEAD OF PURDUE CYTOMETRY DEPT J PAUL ROBINSON
> > PROVIDES MANY GRANTS TO TREESTAR. GRANTS INFORMATION WILL BE PROVIDED
> > AT END…
> > ****READ DO NOT WET YOUR POCKET PROTECTORS***ADDED LINKS TO WEBSITES
> > FOR FLOW JO

> > J.Paul Robinson, PhD PH:(765)4940757> Professor of Immunopharmacology
> > > Professor of Biomedical Engineering
> > > Purdue University FAX:(765)4940517
> > > EMAIL:j...@flowcyt.cyto.purdue.edu
> > > WEB:http://www.cyto.purdue.edu
> > > Have you seen our new HCS webpage?
> > >High Content Screening Website

> > Received on Tue Aug 24 16:18:00 2004
> > * This message: [ Message body ]
> > * Next message: Hans Jürgen Hoffmann: "SV: Any suggestion for
> > selection of ICAM-1 (CD54) antibody?"
> > * Previous message: J. Paul Robinson: "Re: mr on Apple web
> > site"
> > * Maybe in reply to: Prussin, Calman (NIH/NIAID): "mr on Apple
> > web
> > site"
> > * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
> > Subject ] [ By Author ] [ By messages with attachments ]
> > This archive was generated by hypermail 2.1.8 : Wed Aug 25 2004 -
> > 03:12:04 EST
> > *****************************************************************************-
> > ******************************************
> > I know that for a long time, anyone who used a BD flow cytometer had
> > little
> > choice but to use a MAC-data acquisition required it. And, one of
> > the
> > best
> > third party software packages was FlowJo which only ran on a MAC.
> > Mario,
> > being a FlowJock, naturally was heavily exposed to this atmosphere
> > *****************************************************************************-
> > ***************************************
> > For all of the mr groupies out there in cytometry cyberspace. Don't
> > wet> your
> > > > pocket protectors over this.
> > > > Honestly though, well deserved praise for Mario & the Tree Star group:
> > > >Apple - Science - Profiles - Dr. Mario Roederer

> > *****************************************************************************-
> > ********************************************> > Honestly though, well deserved praise for Mario & the Tree Star group:
> > > >Apple - Science - Profiles - Dr. Mario Roederer

> > *****************************************************************************-
> > **********************************************
> > CYTOMETRY SOFTWARE***PURDUE**Links to flow cytometry sites in the USA
> > and Canada (home of Mario Roederer and Steve Perfetto) home of J.
> > Paul
> > Robinson and ...
> > Perfetto, Steve (NIH/VRC) <perfe...@mail.nih.gov>
> > *****************************************************************************¬*
> > GRANTS TROUGH *****PURDUE AND THE GOV. NIH FOR FLOW JO By J.Paul
> > Robinson
> > PRESIDENT OF ISAC 2007 -2008 AND BOARD MEMBER FOR A LONG TIME********
> > The Daily Dongle: - 2 visits - Nov 25
> > Rant about FlowJo's goings-on. ... NIH Funding. Another thread from
> > Purdue worth posting: .... I saw this question on Purdue mailing list
> > earlier today. ...
> > flowjo.typepad.com/the_daily_dongle/2007/06/index.html - 33k - Cached
> > - Similar pages - Note this
> > The Daily Dongle: Current Affairs
> > Rant about FlowJo's goings-on. ... NIH Funding. Another thread from
> > Purdue worth posting:. We are calling upon you again during this
> > Independence Day ...
> > flowjo.typepad.com/the_daily_dongle/current_affairs/index.html - 27k
> > -
> > Cached - Similar pages - Note this
> > [ More results from flowjo.typepad.com ]
> > This page under constructionPUCL Email Archive. Flow cytometry
> > analysis software: ...Tetramer Facility Guide.
> > Ebioscience ...www.immunology.utoronto.ca/research/resources/msb/fc.htm-27k -
> > Cached - Similar pages - Note this
> > The Daily Dongle: - 2 visits - Nov 25Rant about FlowJo's goings-
> > on. ... -the impact of cuts to grants already funded .... I saw this
> > question on Purdue mailing list earlier today. Since FlowJo ...
> > flowjo.typepad.com/the_daily_dongle/2007/06/index.html - 33k - Cached
> > - Similar pages - Note this
> > The Daily Dongle: Current Affairs - 12:10amRant about FlowJo's
> > goings-
> > on. ... For those of you not on Purdue, Joao Loureiro from Portugal
> > wrote: ... -the impact of cuts to grants already funded ...
> > flowjo.typepad.com/the_daily_dongle/current_affairs/index.html - 27k
> > -
> > Cached - Similar pages - Note this
> > Purdue Cytometry Mailing List: By Date - Nov 25Re: Physical
> > exhaustion
> > and immune system FlowJo Technical Support - Maciej Simm (Wed Mar 14
> > 2007 - 17:41:46 ..... Travel grants for young investigators, ...www.cyto.purdue.edu/hmarchiv/Current/thread.htm-288k - Cached -
> > Similar pages - Note this
> > Purdue Cytometry Mailing List: By Date - 2 visits - Nov 25Re: Using
> > DIVA Exp Files in Flojo FlowJo Technical Support - Maciej Simm ......
> > Travel grants for young investigators, ImmunoRio2007
> > gharago...@sums.ac.ir ...www.cyto.purdue.edu/hmarchiv/current/index.htm-318k - Cached -
> > Similar pages - Note this
> > [ More results fromwww.cyto.purdue.edu]
> > The Whitaker Foundation 1996 Annual Report - Grants ApprovedRESEARCH
> > GRANTS Auburn University Thomas S. Denney, Jr., .... State
> > University)
> > Becky Jo Ficek Rice University (B.S. Purdue University) Anne Marie
> > Findlay ...www.bmes.org/WhitakerArchives/96_annual_report/grantsap.html-33k -
> > Cached - Similar pages - Note this
> > Vision now reality in Purdue's Discovery ParkThe park's Birck Center
> > has awarded $2.4 million in start-up grants for nine of those new
> > faculty .... V. Jo Davisson, (7865) 494-5238, davis...@purdue.edu ...www.purdue.edu/UNS/html3month/2004/040919.Rutledge.dispark.html-28k
> > - Cached - Similar pages - Note this
> > Testimony of JO ANNE GOODNIGHTThe development of the miniaturized
> > flow
> > probe marks the first time that anyone has ... Funding provided by an
> > STTR Phase I grant funding resulted in the ...www.hhs.gov/asl/testify/t010621.html-26k - Cached - Similar pages -
> > Note this
> > This page under constructionFlow cytometry users: useful sites.
> > Scripps Research Institute Flow Cytometry Core FacilityTSRI Flow Cytometry Core Facility.
> > Purdue University Cytometry ...www.immunology.utoronto.ca/research/resources/msb/fc.htm-27k -
> > Cached - Similar pages - Note this
> > FACS Flow Cytometry FacilityUse of stand-alone workstation with
> > CellQuest and FlowJo software for data analysis ... Flow cytometry
> > users: useful sitesTSRI Flow Cytometry Core Facility. Purdue ...www.immunology.utoronto.ca/CellSorter.htm-23k - Cached - Similar
> > pages - Note this
> > ISAC Homepage - ISAC E-News -- June, 2007 - 15 visits - Dec 9
> > Best wishes. J. Paul Robinson, President ..... Adam Treister
> > introduced possibilities of integrating these with FlowJo. ...www.isac-net.org/content/view/593/119/-53k - Cached - Similar pages
> > THE PAGE ABOVE IS NOT ACTIVE DUE TO INVESTIGATION OF PURDUE CYTOMETRY
> > MAIL LIST (LINK)
> > ************************************************************************************************************

> > NON READABLE FCS 3 FILES COULTER FC 500 Flow Jo Discussion on Software
> > ERRORS and QUOTING FROM PAUL J ROBINSON HE DOES NOT KNOW IF THEY ARE
> > GOING TO FIX THE PROBLEM OVER THE MAIL LIST>> FLOW JO DISCUSSES
> > ****NEW BUILD OF SOFTWARE FOR CYTOMETRS
> > DOES COULTER KNOW? THIS IS COULTERS SOFTWARE USED IN THE FC 500
> > CYTOMETER?
> > **************************************************************************************

> > RE: Nonreadable FCS 3 files from Coulter FC 500 cytometer
> > * This message: [ Message body ] [ More options ]
> > * Related messages: [ Next message ] [ Previous message ] [ In
> > reply
> > to ]
> > From: J. Paul Robinson <j...@flowcyt.cyto.purdue.edu>
> > Date: Fri Jun 04 2004 - 21:02:48 EST
> > We notified Coulter of the error in their software a
> > long time ago. I dont know if htey are going to fix it.
> > regards
> > paul robinson
> > On 3 Jun 2004 at 16:55, maciej simm wrote:

> > - Hide quoted text -

> > > We are currently working on adding the support of these files in the Windows
> > > version of FlowJo, and we notified Coulter of our findings in our efforts so
> > > far. There is a keyword $PnB which specifies the byte length for the parameter
> > > "n". In the LMD files in question, this value is seems to be interfering with
> > > the interpretation of this data by software which requires strict FCS
> > > compliance.
> > > I don't want to start a big debate over standards (ok, how about a medium
> > > one?) but life would be a lot easier for all FCS analysis software
> > > manufacturers if the data would be written in a consistent way by all
> > > manufacturers who develop acquisition software.
> > > I will post again once we've got a build of FlowJo for Windows that supports
> > > this format.
> > > I'm also looking forward to the utility Paul mentioned in an email earlier
> > > today. I couldn't find it on CD8, what is it called? I looked in the
> > > /content/software folder.
> > > Maciej Simm
> > > Tree Star Inc.
> > > Technical Support
> > > > -----Original Message-----
> > > > From: Tomas Kalina [mailto:tomas.kal...@lfmotol.cuni.cz]
> > > > Sent: Wednesday, June 02, 2004 4:13 AM
> > > > To: Cytometry Mailing List
> > > > Subject: Nonreadable FCS 3 files from Coulter FC 500 cytometer
> > > > Hello,
> > > > we are running a cooperative minimal residual disease study with people
> > > > using different platforms (BD and Coulter). For data analysis we want to
> > > > use a centraly designed templates made in Flow Jo software.
> > > > Unfortunately our colleague's Coulter-FC 500 cytometer is producing FCS
> > > > 3 files that are not readable by any other software (Flow Jo or
> > > > CellQuest).
> > > > The troubled instrument is Coulter FC 500 with the Cytomics RXP software. ********
> > > > Doeas anybody have a suggestion how to overcome the Coulter FCS 3 *****
> > > > incompatibility?
> > > > Thank you Tomas Kalina
> > > > --
> > > > Tomas Kalina,M.D.
> > > > Institute of Immunology
> > > > Charles University - 2nd Faculty of Medicine
> > > > tomas.kal...@centrum.cz
> > > > fax (+420) 22443 5962
> > > >http://www.lf2.cuni.cz/clip/
> > > > phone (+420) 22443 5968, (+420) 22443 5969, (+420) 22443 2084
> > > > postal address: V uvalu 84
> > > > 150 06 Praha 5
> > > > Czech Republic

> > J.Paul Robinson, PhD PH:(765)4940757 ***********
> > Professor of Immunopharmacology
> > Professor of Biomedical Engineering
> > Purdue University FAX:(765)4940517
> > EMAIL:j...@flowcyt.cyto.purdue.edu
> > WEB:http://www.cyto.purdue.edu
> > Have you seen our new HCS webpage? ***************High Content Screening Website
> > Received on Mon Jun 7 14:18:00 2004
> > * This message: [ Message body ]
> > * Next message: Donnenberg, Albert: "RE: Nonreadable FCS 3 files
> > from
> > Coulter FC 500 cytometer"
> > * Previous message: Michael Ormerod: "Flow Cytometry Course"
> > * In reply to: maciej simm: "RE: Nonreadable FCS 3 files from
> > Coulter
> > FC 500 cytometer"
> > * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
> > Subject ] [ By Author ] [ By messages with attachments ]
> > This archive was generated by hypermail 2.1.8 : Tue Jun 08 2004 -
> > 03:12:04 EST
> > FLOW JO AND MARKET SHARE DISCUSSION VS BD
> > MARKET SHARE
> > *****************************MARKET SHARE DISCUSSION DOES BD KNOW?
> > ***********
> > **************************************************************************************************************************
> > Of course, what started this thread was the assertion in the piece
> > about
> > Mario on Apple's web site that there were 15,000 to 20,000 Mac-based
> > flow
> > cytometers out there. The best estimates I had from industry people
> > in
> > early 2003 when I was finishing up the 4th Edition of PFC was that
> > the
> > total number of systems from all manufacturers in use was under
> > 20,000. If
> > that's correct, BD would need a 75% market share to account for the
> > low end
> > estimate of 15,000 Mac-based machines. I'd be interested to know
> > where
> > the
> > 15,000 to 20,000 figure came from.
> > *****************************************************************************¬***********************************************
> > Re: mr on Apple web site
> > * This message: [ Message body ] [ More options ]
> > * Related messages: [ Next message ] [ Previous message ] [ In
> > reply
> > to ] [ Next in thread ] [ Replies ]
> > From: Howard Shapiro <h...@shapirolab.com>
> > Date: Wed Aug 25 2004 - 18:15:38 ESTRobin Barclay wrote:
> > >Well .... some of us PC afficianados hate Macs just as much - personally I
> > >have hated them since they first made it difficult to access their DOS and
> > >write programs in any language (especially for accessing/controlling lab
> > >apparatus) - back in the '70's when there were several different options -
> > >not just PC's. I am glad that the "PC" became a standard (there were too
> > >many diferent systems) and that IBM did not hang on to it the way Apple kept
> > >the Mac in house (you get more for your money with a PC because many
> > >different people make them). You don't often get PC people knocking Macs
> > >the way that the Mac people knock PCs - there seems to be a lot of Microsoft
> > >paranoia. In my opinion PCs are much more common and versatile in labs than
> > >Macs - especially outside the USA - and will eventually become the standard
> > >for interfacing with lab equipment..... and they can "look cool" if you shop
> > >around for the right case if that's important to you.

> > I can't pass up the opportunity to get into the PC/Mac battle - I
> > hate
> > them
> > both, but there aren't really alternatives (yes, I know, there's
> > Linux, but
> > it's probably even harder to hook up a Linux system to hardware than
> > it is
> > to hook a Mac up to hardware).
> > Of course, what started this thread was the assertion in the piece
> > about
> > Mario on Apple's web site that there were 15,000 to 20,000 Mac-based
> > flow
> > cytometers out there. The best estimates I had from industry people
> > in
> > early 2003 when I was finishing up the 4th Edition of PFC was that
> > the
> > total number of systems from all manufacturers in use was under
> > 20,000. If
> > that's correct, BD would need a 75% market share to account for the
> > low end
> > estimate of 15,000 Mac-based machines. I'd be interested to know
> > where
> > the
> > 15,000 to 20,000 figure came from.
> > The old (68000 series and possibly early PowerPC) Macs were
> > difficult,
> > but
> > not impossible to connect to hardware; it was easier to work with the
> > PC's
> > ISA bus, which, while slow, was perfectly adequate to do most flow
> > cytometric data acquisition and analysis. There were decent versions
> > of
> > Forth, which was one of the first and best languages designed for
> > controlling hardware from mini- and microcomputers, for both PC (DOS)
> > and
> > the Mac (Forth was the first Mac programming language made
> > commercially
> > available, at a time when the only other option was buying the very
> > expensive Lisa from Apple on which to develop Mac software). I found
> > MacForth easier to program with than the Windows Forths (or other
> > Windows
> > languages, e.g., Delphi), but my old Macs used to crash all the time.
> > So what makes me unhappy with both Windows [and Windows machines] and
> > the
> > Mac in their current incarnations? It is now significantly harder for
> > mere
> > mortals to write software to get hardware to communicate with either
> > PCs or
> > Macs; the gain in complexity associated with the PCI bus, USB/USB2,
> > FireWire(IEEE 1394), etc. is greater than the gain in speed and
> > convenience. Also, in making the operating systems more stable (and
> > Win XP,
> > despite its security issues, is almost as stable as Mac's Unix-based
> > OS X),
> > both Microsoft and Apple elected to eliminate the ability of their
> > computer
> > hardware to respond rapidly to interrupts (latencies are now in the
> > tens of
> > thousands of instruction cycles), meaning that any really fast
> > hardware
> > attachment for either a PC or a Mac now needs to have a DSP in it,
> > whereas
> > if the fast interrupt response had been preserved, the hardware
> > attachments
> > could have been made much simpler and cheaper. Linux also takes fast
> > interrupt response off the table, so it doesn't represent a viable
> > alternative. If you go to Apple's web site and look at what data
> > acquisition hardware is available for Macs, particularly for Macs
> > running
> > OS X, there isn't much, and many of the companies that supported the
> > Mac in
> > that area have dropped Mac support for their newer products. That is
> > undoubtedly one big reason why BD's newer digital pulse processing
> > cytometers are running on the PC platform.
> > That doesn't stop anybody from analyzing FCS files on Macs. FlowJo is
> > well-conceived flow cytometry software; one reason it is as good as
> > it
> > is
> > is that it was written by people who do a lot of flow cytometry, and
> > cutting edge flow cytometry at that. But there are other people who
> > do
> > a
> > lot of good flow cytometry who have written good software, for PCs as
> > well
> > as for Macs.
> > For the record, I have a G4 PowerBook, which I use mostly for the
> > iLife
> > applications, which are slick. It doesn't crash more than once every
> > couple
> > of months, but the same is true of my Windows 2000 and XP systems. OS
> > X can
> > be as infuriating as Windows when one or another aspect of it goes
> > counter
> > to your intuition or to what you have gotten used to. Macs, while
> > somewhat
> > more expensive, are much better made than many PCs, and they are
> > certainly
> > aesthetically pleasing. If there were a reasonable alternative to
> > Microsoft's Office applications for the Mac, I might consider
> > switching.
> > The 12.1" PowerBook is a nice portable, but it's over a pound heavier
> > than
> > the Fujitsu laptop I now use, which has pretty much the same speed,
> > memory,
> > and drive capabilities (OK, not DVD-R, but I don't burn a lot of
> > DVDs).
> > However, I really wish Apple had stuck with the plan they had a few
> > years
> > back of writing a Mac operating system for Intel hardware. I think
> > that
> > died when Microsoft bailed Apple out with a few hundred mil. If it
> > were
> > possible to run OS X and XP mano a mano on the same hardware, there'd
> > be a
> > more rational basis for comparison. But, as may be the case for the
> > November election, minds, once made up, are not easily changed.
> > -Howard
> > Received on Thu Aug 26 16:38:00 2004
> > * This message: [ Message body ]
> > * Next message: Konz, Richard: "Discrimination of GO/G1"
> > * Previous message: Ann Atzberger: "RE: optimum concentration
> > for
> > sorting"
> > * In reply to: Robin Barclay: "Re: mr on Apple web site"
> > * Next in thread: Robert C. Leif: "RE: mr on Apple web site"
> > * Reply: Robert C. Leif: "RE: mr on Apple web site"
> > * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
> > Subject ] [ By Author ] [ By messages with attachments ]
> > This archive was generated by hypermail 2.1.8 : Sat Aug 28 2004 -
> > 03:12:06 EST
> > HOW TO DEVELOP SOFTWARE? YOU CAN USE EXISTING COMMERCIAL PROGRAMS?
> > RESPONSE WITH FLOW JO ***ADAM TRIESTER TREESTAR/FLOW JO
> > **************************************************************************************************************************
> > You can use existing commercial programs (Aabel & JMP are nice
> > Mac 3D applications, and I'm sure there are others), or write it
> > yourself in OpenGL. I think OpenGL would be a better choice than C+
> > +.
> > OpenGL is a higher level graphics language, and knows how to access
> > the
> > specialized accelerators in the graphics cards, which are actually
> > faster for this stuff than the G5. That's how the dungeon games do
> > the
> > 3D shading and rendering.
> > I'll be happy to donate a considerable amount of code that I've done
> > in
> > this effort (most was taken from an old freeware program called
> > Rotator, which I no longer could find with Google, but I have
> > somewhere
> > in my archives), but we decided this was pretty much a dead end.
> > Rotator was in C, and quite unreadable. For the investment this task
> > would take, I think it'd be better to start over in OpenGL. It's
> > cross-platform too, which is important, as you'll find you want to
> > port
> > it to a Cray.
> > I'd still think you want to use FlowJo to read the DiVa files,
> > compensate them, transform them, gate them, and then export desired
> > subpopulations to the 3D viewer. If it were any other instrument,
> > you
> > could probably read the files yourself, with R or our free Java
> > libraries, but the DiVa files are a unique format, and almost always
> > require compensation and transformation to a lin/log scale, so
> > there's
> > a lot of work before you even get to viewing them.
> > *****************************************************************************-
> > ***********************************************
> > Re: 3D Graphics for flow data display
> > This message: [ Message body ] [ More options ]
> > Related messages: [ Next message ] [ Previous message ] [ Maybe in
> > reply to ] [ Next in thread ]
> > From: Adam Treister <a...@treestar.com>
> > Date: Sun Jan 04 2004 - 13:48:24 EST
> > David,
> > We've tried on a couple of occasions to add a "spatial" 3D module to
> > FlowJo, and it has never turned out well enough to make it into a
> > release. We've found that using time as the third dimension, as in
> > our
> > "data movies," or using several 2D graphs, as in our "multi-graph
> > overlay" to be more practical solutions.
> > If you want smoothing or density coloring, that requires binning the
> > data. Even working at low resolution, you're looking at 256 times as
> > much time and memory to take the plot into an additional dimension.
> > You might get a tenfold performance increase with the G5 (which I
> > think
> > is quite optimistic, because the G5 adds fast floating point
> > processing, but binning is a integer operation), but even with that
> > that, adjusting a gate goes from taking perhaps a second to almost a
> > half minute. That would make using FlowJo feel like using CellQuest
> > (just kidding ;) At the full resolution of DiVa files, you're
> > looking
> > at another thousand fold increase over the 2D version, or a billion
> > times (1000 ^ 3) as long as we take to do it now. So, as best I
> > can
> > figure it, we can only support 3D at the cost of losing interactivity
> > with the data (ie, we can make the views, but changing gates or
> > parameters won't immediately change the 3D visualization).
> > It would be tough to have contours in 3D as each layer would obscure
> > the ones inside it. Contours would have to have varying opacity,
> > which
> > not only increases the computational time and complexity, but would
> > make it hard to differentiate populations. And the user interface
> > for
> > gating in space would be a real challenge. You could chop thru space
> > with planes, but that's 1D gating, which doesn't give you more
> > capability to define populations than you have now. So we'd have to
> > invent polyhedral gating.
> > If all you want to do is look at already-gated populations in 3D,
> > there
> > are options that exist. Expo32 has this feature, if you can figure
> > out
> > how to use Beckman Coulter (actually, ACS wrote it) software to view
> > BD
> > files. You can use existing commercial programs (Aabel & JMP are
> > nice
> > Mac 3D applications, and I'm sure there are others), or write it
> > yourself in OpenGL. I think OpenGL would be a better choice than C+
> > +.
> > OpenGL is a higher level graphics language, and knows how to access
> > the
> > specialized accelerators in the graphics cards, which are actually
> > faster for this stuff than the G5. That's how the dungeon games do
> > the
> > 3D shading and rendering.
> > I'll be happy to donate a considerable amount of code that I've done
> > in
> > this effort (most was taken from an old freeware program called
> > Rotator, which I no longer could find with Google, but I have
> > somewhere
> > in my archives), but we decided this was pretty much a dead end.
> > Rotator was in C, and quite unreadable. For the investment this task
> > would take, I think it'd be better to start over in OpenGL. It's
> > cross-platform too, which is important, as you'll find you want to
> > port
> > it to a Cray.
> > I'd still think you want to use FlowJo to read the DiVa files,
> > compensate them, transform them, gate them, and then export desired
> > subpopulations to the 3D viewer. If it were any other instrument,
> > you
> > could probably read the files yourself, with R or our free Java
> > libraries, but the DiVa files are a unique format, and almost always
> > require compensation and transformation to a lin/log scale, so
> > there's
> > a lot of work before you even get to viewing them.
> > I've promised you 3D graphs in FlowJo in the past, and I've done my
> > best to deliver them, but the results have been pretty
> > disappointing.
> > And the benefit of them has never been demonstrated. If you can show
> > us how 3D views provide more interpretable data than our current
> > "compromise solutions," that would help. If you want to pick a
> > data
> > file, we'll make you get a spinning, stereoscopic, 3D view of it. If
> > we find that other scientists are able to make conclusions about the
> > data better than they can from our existing visualizations, that will
> > go a long way towards bumping it up on the FlowJo future feature
> > list.
> > I hope that helps.
> > Adam
> > On Dec 31, 2003, at 8:38 AM, David Dombkowski wrote:
> > - Hide quoted text -
> > - Show quoted text -

> > - Hide quoted text -

> > > I am addressing this inquiry to all who may have suggestions as to
> > > software tools that will be of aid in achieving my goal.
> > > I wish to produce software for the Macintosh G5 computer running OS X
> > > that will allow for the display of 3 dimensional plots to aid in the
> > > analysis of 10 or more color data. Displaying this data in only 2
> > > dimensional plots is clearly limiting. My goal is to be able to
> > > display high resolution DiVa data in 3 dimensional plots that can be
> > > manipulated so as to allow for various viewing angles in real time.
> > > Memory will not be limited so that this will not be a limiting factor.
> > > I believe the best code for this software will be C ++.
> > > Please feel free to respond to this inquiry publicly so that we may
> > > have a discussion as well as collaboration on achieving this gaol. The
> > > time has come to develop this software and distribute freely among
> > > those who see the potential of such an application.
> > > David
> > > --
> > > David M. Dombkowski
> > > dombkow...@helix.mgh.harvard.edu
> > > Flow Cytometry-Pathology-CNY rm7017
> > > Massachusetts General Hospital-East
> > > 149 13th Street
> > > Charlestown, MA 02129
> > > Tel (617)-726-1683
> > > Fax (617)-724-3164

> > Received on Mon Jan 5 13:58:00 2004
> > This message: [ Message body ]
> > Next message: facs_c...@wehi.EDU.AU: "Re: 3D Graphics for flow data
> > display"
> > Previous message: Joost Schuitemaker: "intracellular staining of ...
> > 2004"
> > Maybe in reply to: David Novo: "Re: 3D Graphics for flow data
> > display"
> > Next in thread: facs_c...@wehi.EDU.AU: "Re: 3D Graphics for flow data
> > display"
> > Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
> > Subject ]
> > [ By Author ] [ By messages with attachments ]
> > This archive was generated by hypermail 2.1.8 : Thu Jan 22 2004 -
> > 12:12:46 EST
> > VERITY SOFTWARE HOUSE INC. MARKITING AND SALES ON LIST
> > ****************************************************************************************
> > Re: DNA analysis software
> > * This message: [ Message body ] [ More options ]
> > * Related messages: [ Next message ] [ Previous message ] [ In
> > reply to ]
> > From: Xiaoping Wu <xiaopi...@psbc.org>
> > Date: Thu May 24 2007 - 17:04:13 EDT
> > We have Modfit 3.0 and our computer is installed with both OS 9 and
> > OS
> > X. We did not find problem with printing.
> > On May 23, 2007, at 2:33 PM, Turbov, Jane wrote:

> > - Hide quoted text -

> > > Hello Flow-ers,
> > > I would like to revisit the question of DNA analysis. We have ~ 12
> > > year old FacsCalibur and have just upgraded our system to Mac Os X. We
> > > had ModFit LT 2.0 installed previously. We are having problems
> > > installing Mac Classic but hopefully this will be resolved soon. From
> > > the Verity website, it appears that ModFit 3.0 can run off of Mac OS X
> > > but printing may be an issue.
> > >Knowledge Base Detail
> > > Our lab manager suggested FLOJO
> > >http://www.flowjo.com/specproliferation.htmlbutthis might be too
> > > sophisticated for our needs.
> > > Does anyone have any recommendations?
> > > Thanks,
> > > Jane Turbov
> > > Department of OBGYN Research
> > > ENH Research Institute
> > > 2650 Ridge Avenue
> > > Evanston, IL 60201
> > > TEL: 847-570-4021
> > > FAX: 847-733-5256
> > > From: VSH Tech Support [mailto:T...@vsh.com]
> > > Sent: Wednesday, February 21, 2007 10:30 AM
> > > To: Cytometry Mailing List
> > > Subject: RE: DNA analysis software
> > > Hello Ibtissam,
> > > ModFit LT, for PC or Mac, has advanced modeling capability for
> > > research applications in DNA cell cycle analysis. You may use any of
> > > the model templates the program offers, or create your own models for
> > > non-traditional analysis, including non-mammalian DNA cell cycle
> > > studies. ModFit LT can be linked to our WinList program to provide a
> > > complete cell cycle analysis on any number of sub-populations with a
> > > single click of a button.
> > > For an overview, visithttp://www.vsh.com/products%a0.
> > > Best regards,
> > > Don
> > > Donald J. Herbert
> > > Technical Support Manager
> > > Verity Software House
> > > ________________________________
> > > From: Ibtissam Abdul-Jabbar [mailto:iajab...@cicr.uq.edu.au]
> > > Sent: Wednesday, February 14, 2007 11:41 PM
> > > To: cyto-inbox Subject: DNA analysis software
> > > Dear All, before buying software to analyse DNA on PC, I would like
> > > to get your opinion. I already have ModFit for Macintosh.
> > > What are you using and what do you recommend for research purposes.
> > > Is MultiCycle Av the one of choice?
> > > I appreciate your comments.
> > > Ibtissam A Jabbar (PhD)
> > > Manager of the FACS facilities
> > > Diamantina Institute for Cancer, Immunology and Metabolic Medicine
> > > (DI)
> > > The University of Queensland
> > > Level 4 R Wing
> > > Princess Alexandra Hospital
> > > Ipswich Rd Buranda QLD 4102
> > > Australia
> > > Ph: 07 3240 5945
> > > Fax: 07 3240 5946
> > > Mob: 0401154744

> > Xiaoping Wu, Ph.D.
> > Flow Cytometry Laboratory
> > Research Division of the Puget Sound Blood Center
> > 1201 Ninth Avenue
> > Seattle, Washington 98101
> > *****************************************************************
> > This e-mail and any attachments may contain confidential and
> > privileged information. It has been scanned for viruses. If you
> > are not the intended recipient, please notify the sender
> > immediately by return e-mail, delete this e-mail and destroy any
> > copies. Any dissemination, use, review, disclosure, or
> > distribution of this information by a person other than the
> > intended recipient is unauthorized and may be illegal.
> > *****************************************************************
> > Received on Fri May 25 13:38:00 2007
> > * This message: [ Message body ]
> > * Next message: Darzynkiewicz, Zbigniew: "RE: Chromatin Structure
> > Aassay"
> > * Previous message: Derek Davies: "Re: Fat-binding colours??"
> > * In reply to: Turbov, Jane: "RE: DNA analysis software"
> > * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
> > Subject ] [ By Author ] [ By messages with attachments ]
> > This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 -
> > 03:12:00 EST
> > VERITY SOFTWARE HOUSE COMPARES SOFTWARE NOT RELEASED WITH BD SOFTWARE
> > ****************************************************************************************
> > Purdue Cytometry Mailing List: Analysis software for the Mac
> > ... Purdue Cytometry Mailing List: Analysis software for the Mac ...
> > programs divide into two price categories, the expensive (CellQuest,
> > MacLAS, FCAP List, and Verity's WinList for the Mac, not yet
> > released)
> > and the relatively ...http://www.cyto.purdue.edu/hmarchiv/1997/2083.htm-5.8KB 57%
> > ||||||||||||||||||||
> > 08 Sep 97
> > Find Similar
> > Highlight

> > EVERY SOFTWARE DEVELOPER AND CORPORATE OWNER ON THE MAIL LIST ABOVE
> > HAS LICENSE AGREEMENTS ON SOFTWARE…ALL SOFTWARE MUST BE LICENSED….
> > I DO NOT KNOW IF PURDUE GETS ROYALTIES FROM ALL THE PROVIDERS ABOVE
> > BUT THE MAIL LIST WAS UNDER TIGHT PROTECTION BY PAUL J. ROBINSON ISAC
> > PRESIDENT HEAD OF PURDUE CYTOMETRY
> > **************************************************************************************

> > KANECKI ASSOCIATES INC. INTRODUCED A NEW CYTOMETRY SOFTWARE WITH NO
> > LICENSE FEE AT A LOW COST TO HELP STUDENTS AND PROGRESS… THE SOFTWARE
> > IS THE ONLY SOFTWARE THAT DOES NOT USE COMPENSTAION AND CAN PROCESS 10
> > MILLION EVENT FILES BY 40 PARAMETERS.
> > *************NO LICENSE FEES AND ONLY $150.00 FOR STUDNETS!
> > *************

> > HERE IS HOW THE DISCOVERY OF THE MAIL LIST CAME ABOUT…

> > FLOW CYTOMETRY SOFTWARE DEVELOPER KANECKI ASSOCIATES INC. WAS CALLED
> > A
> > SCAM BY PERDUE J PAUL ROBINSON...THE STORY AND COVER UP THAT IS
> > WITHIN
> > THE SYSTEM..WILL THE TRUTH COME OUT?
> > KANECKI ASSOCIATES INC.INTRODUCES NEW FCS CYTOPRO QUICK FACS 11 TO
> > PERDUE UNIVERSITY ONLY TO BE INSULTED WITHOUT REASON... UNCOVER THE
> > SCANDLE THAT IS WITHIN THE SYSTEM AND SEE IF THERE IS A CHANCE FOR
> > REFORM? WILL IT EVER CHANGE? IT MUST COME TO A END
> > Nov 22, 2007 16:38
> > Flow Cytometry Software Developer Kanecki Associates Inc. Gets Called
> > A SCAM by PERDUE's J PAUL ROBINSON
> > Posted by cytometryuser
> > Kanecki Associates Inc. Was SHOCKED when J.Paul Robinson from PERDUE
> > called them SCAMMERS after being Questioned from the University about
> > the Response about his prior comment of them sending JUNK MAIL to his
> > Mail List...THE MAIL LIST seemed to be a very sensitive spot for Mr.
> > Robinson....
> > After a email was sent by a undisclosed source with a link providing
> > a
> > link to the MAIL LIST everything was uncovered FAST....The link was a
> > open door to a list of MAIL FROM a group of HIGH POWER
> > INDIVIDUALS...MOST...SOFTWARE PROGRAMMERS THAT DEVELOPED SOFTWARE
> > WHILE GETTING EDUCATED THROUGH PURDUE...
> > KANECKI ASSOCIATES INC. WOULD LIKE TO STATE THEY ARE NOT SCAMMERS NOR
> > DID THEY EVER ATTEMPT TO GET ON THE PERDUE MAIL LIST...They have
> > never
> > sent mail through the list but just tried to introduce the NEW
> > software that is distributed through C/net, Best Buy, Circuit City,
> > Simtel, and may other Major Down load sites.
> > To view the software JUST GOOGLE: FCS CYTOPRO QUICK FACS 11.
> > KANECKI ASSOCIATES INC. RELEASED THE NEW FCS CYTOPRO JAVA 6.0 FOR
> > DOWNLOAD TOO.
> > **********************WITH NO LICENSE
> > FEES******************************** AND REASONABLE PRICES THEY
> > DELIVER A SUPERIOR PRODUCT THAT PROCESS 10 MILLION EVENT FILES...FCS
> > 3.0
> > THEY DO NOT USE COMPENSATION LIKE OTHER SOFTWARE PROVIDERS SO THERE
> > IS
> > NO OVER COMPENSATION ISSUES........Further More there is NO MENUE BAR
> > which makes for USER FRIENDLY SOFTWARE...VERY EASY TO USE....JUST
> > PICK
> > AND CLICK.................................
> > SO TO PROVIDE THE TRUTH ALL POST THAT WILL BE SHOWN CAME DIRECTLY
> > FROM
> > SAVED EMAIL SENT TO PURDUE...IT WAS NOT SENT TO THE LIST BUT TO THE
> > HEAD OF PURDUE..
> > NOT MR.ROBINSON HE RECEIVED A CC...THEN RESPONDED BU CALLING IT JUNK
> > MAIL!
> > ORIGINAL MESSAGE SENT : TO ROBERT MURPHY WITH LINKS FOR
> > DOWNLOAD AND ALL INFORMATION PERTAINING TO FCS CYTORPO QUICK FACS 11
> > SOME HOW IT WAS FOWARDED TO: J PAUL ROBINSON...MAYBE FOR REVIEW...?
> > Fw: NEW FLOWCYTOMETRY SOFTWARE FOR ALL BD and Beckman/Coulter
> > Flow Cytometers CAN PROCESS 24,000 SAMPLES PER HOUR
> > Standard
> > Header|Full Message
> > View
> > mitchell haynes <buybro...@yahoo.com>
> > ViewFriday,
> > September 28, 2007 1:36:36 AM
> > To:j...@flowcyt.cyto.purdue.edu
> > Cc:da...@kanecki2.com
> > Cytopro
> > quick facs savings analysis.doc (82KB); Setup-Quick
> > Facs 11 Demo.exe (7854KB); FCS
> > CYTOPRO BENIFITS.doc (25KB)
> > Find:
> > Dear Robert Murphy
> > NEW
> > FLOWCYTOMETRY SOFTWARE FOR ALL BD and Beckman/Coulter Flow Cytometers
> > CAN
> > PROCESS 24,000-42,000 SAMPLES PER HOUR.
> > FLOW CYTOMETRY FCS CYTO PRO QUICK FACSKanecki Associates - The Future of Software Technology and World Leader in Intelligent Thinking Systems Management Government Military Intellectual Property
> > Increased quality and productivity. With 10,000,000 event files, you
> > can process 24,000 samples/hour, and maintain quality up to Sigma 5
> > or
> > better. Compare this to having your research technologist performing
> > only 100 samples/hour analysis.
> > Increased laboratory utilization by 3X because you can perform the
> > analysis off-lab and free laboratory time for reading samples. This
> > was achieved when I developed the program, and we had a program
> > project grant from 1992 to 1998 of $8M.
> > Works with FCS 3.0 in all data modes as floating point, integer*4,
> > and
> > ASCII.
> > Works with BD and Beckman/Coulter Flow Cytometers and Cell Sorters
> > Backwards compatible with FCS 2.0 files and Flow Cytometers and Cell
> > Sorters.
> > Can read FCS 3.0 files up to 10M events with 20 parameters.
> > Easy to Use, three step process. Load initial file, set gate, specify
> > file list to process. That's it.
> > Collaboration tools to allow you to cut and paste image results to
> > results.
> > Statistical analysis results imprinted on histogram plots directly as
> > mean, mode, and median with the ability to present results in log
> > mode
> > or linear mode, depending on the detector used.
> > Plain vanilla coding for easy update and maintenance to allow for the
> > greatest user and software quality.
> > One time purchase fee, no yearly renewal fees as with others.
> > Proven tract record in FACS, Fluorescent Activated Cell Sorter
> > Laboratory. The laboratory was rated the best laboratory in the
> > Midwest USA in 1990.
> > This application is designed for large-scale fluorescent activated
> > cell sorter
> > analysis. The program can read up to FCS 3.0 files and has been
> > tested
> > to run on Becton Dickinson and CoulterOrtho based flow cytometers and
> > cell sorters. The main advantage of this program is that you can have
> > the computer perform the analysis for you after you have selected the
> > region to analyze. The result is that up to 24,000 samples per hour
> > can be analyzed on a 1.4 GHz speed computer.
> > This program is designed for researcher and technologist use. It uses
> > rectangular gating, and is intuitive to use. To use this program, the
> > FCS must have the extension, *.bin as "54203023.bin" as an example.
> > The *.bin extension is what the computer uses to locate the files on
> > the computer.
> > THANK YOU FOR YOU TIME
> > MITCHELL HAYNES
> > VP
> > SALES
> > KANECKI
> > ASSOCIATES
> > 832-347-1669
> > THIS WAS J PAUL ROBINSON RESPONSE TO THE INFORMATION SENT
> > Re: Fw: NEW FLOWCYTOMETRY SOFTWARE FOR ALL BD and Beckman/Coulter
> > Flow
> > Cytometers CAN PROCESS 24,000 SAMPLES PER HOUR...Standard Header|Full
> > Message View J. Paul Robinson J. Paul Robinson ... ViewFriday,
> > September 28, 2007 9:37:32 AM To:mitchell haynes
> > Cc:da...@kanecki2.com; skel...@flowcyt.cyto.purdue.edu
> > Steve
> > what is this email - it came to me with Bob Murphy's name associated
> > with it. It seems to be an advertisement, this junk mail, and it
> > seems
> > to have been modified by you ...
> > So I guess I am confused. was this sent to the list, or do you have
> > an
> > details about it - i am concerned about these junk messages going out
> > to
> > our members, - if they are using our lists, I will deal with them
> > appropriately, but I am not happy about this - any info you can give
> > me
> > appreciated
> > thanks
> > paul
> > WHILE BEING SHOCKED BY THIS MAIL...MITCHELL HAYNES VP.KANECKI
> > ASSOCIATES INC....WROTE BACK
> > : mitchell haynes <buybro...@yahoo.com>

> > - Hide quoted text -

> > > To: skel...@flowcyt.cyto.purdue.edu
> > > Cc: skel...@flowcyt.cyto.purdue.edu
> > > Sent: Friday, September 28, 2007 11:39:02 AM
> > > Subject: NOT A JUNCK MAIL STEVE WAS JUST FOWARDING INFORMATION TO PROPER
> > > CHANNELS
> > > Dear Paul,
> > > I recieved your responce to the email I sent. Please understand it is not
> > > junkmail but a update on new technology that will inhance all
> > > flowcytometers..It is currently being evaluated by BD who request for this
> > > software to be developed directly by our corporation.
> > > It was simply sent as an announcement for you concideration.
> > > The software is demonstrates precision and a higer processing rate than
> > > every existing software today.
> > > If you have any questions please call I provided my phone number with the
> > > email. I understand institutions of your caliber is always looking for new
> > > technology. Futhermoore this is the only software in the world that works
> > > for every platform on one peice of software
> > > Thank your for you time and have a great day.
> > > Please do not blame Steve for send the information to the proper channels
> > > I would think you would be upset if he did not foward important infomation
> > > that pertains to furthering cytometry breakthroughs.
> > > If you would like us to send information to another address that won't
> > > interfer please foward it to me and I will make sure that there are no
> > > more misunderstandings.
> > > Mitchell Haynes

> > <!--[if !supportLineBreakNewLine]-->
> > AFTER THAT MESSAGE J PAUL ROBINSON RESPONDED WITH OUT REASON!

> > - Hide quoted text ------ Original Message ----
> > From: "j...@flowcyt.cyto.purdue.edu"

> > <j...@flowcyt.cyto.purdue.edu>
> > To: mitchell haynes <buybro...@yahoo.com>
> > Cc: j...@flowcyt.cyto.purdue.edu
> > Sent: Friday, September 28, 2007 2:26:51 PM
> > Subject: Re: Fw: NOT A JUNCK MAIL STEVE WAS JUST FOWARDING
> > INFORMATION
> > TO
> > PROPER CHANNELS
> > Sorry, I think it is junk mail
> > regards
> > paul robinson
> > After this message Kanecki Associates Inc. JOINED many Forums in
> > which
> > the MESSAGES WITH THE NAME OF THE SOFTWARE AS RELAYED.....JUST ABOUT
> > ALL FORUMS DELETED INFORMATION POSTED ABOUT THE
> > SOFTWARE...FURTHERMOORE...BEING REMOVED AND BANNED FROM FORUMS....
> > VERY CONFUSED ABOUT ALL THE TALK OF OTHER SOFTWARE THE LAUNCHED
> > QUESTIONS ON YAHOO..?
> > WHY DO FORUMS ALLOW THE CONVERSATION OF ONE SOFTWARE AND NOT
> > ANOTHER..
> > WHY DO FLOW CYTOMETRY COMPANIES CHARGE LICENSE FEES?
> > THEN SOMEONE SENT A LINK TO THE PURDUE MAIL LIST BUT IT WAS NOT TO
> > THE
> > FRONT BUT BACK ROUTE..WHEN VIEWED ALL THE REASONS WERE EASY TO
> > SEE...........
> > J PAUL ROBINSON HAD A LOT OF POWER AND INFLUENCE IN THE FLOW
> > COMMUNITY...
> > FURTHER MOORE EVERY PROGRAMMER THAT HAS SOFTWARE ON THE MARKET WENT
> > TROUGH THE MAIL LIST SINCE 1992 AND ALL HAD THEIR OWN SOFTWARE FOR
> > SALE ON THE PERDUE SITE.....THE POST IN THE MAIL LIST ARE UNFILTERED
> > AND WERE******* NOT WHAT THE REST OF THE LIST HAD ACCESS TO...ONCE
> > POST STARTED GOING UP THE SITE WAS BLOCKED... SINCE THEN BOTH SITES
> > HAVE BEEN UP AND DOWN FOR THE MAIL LIST!
> > WITH THE MARKET BEING VERY SMALL IN FLOW SOFTWARE IT IS EASY TO
> > CONTROL....
> > KANECKI ASSOCIATES POSTED A BLOG WITH THE LINK TO THE BACK ROUTE TO
> > THE MAIL LIST AND GOT IMMEDIATE RESPONCE...
> > WHEN MITCHELL HAYNES DISCOVERED THE LINK HE STARTED POSTING THE
> > DISCOVERY UP ON MIS.HEALTH AIDS THROUGH :::GOOGLE::: AS FOR*****
> > GOOGLE*****IS THE ONLY SITE NOT FILTERING THE MAIL....
> > FINALLY A THE MESSAGES WERE SEEN AND SINCE THEY CAME FROM THE MAIL
> > LIST POSTINGS THEY WERE NOT PRETTY....
> > ALL FILES WERE DOWNLOADED
> > WHAT CAN KANECKI ASSOCIATES INC FCS CYTOPRO JAVA 6.0 DO? NO LICENSE
> > FEE
> > 0NLY $150.00 FOR STUDENTS 250.00 USD EVERYONE ELSE
> > ***********************************************************************************************************************
> > POSTED BY MITCHELL HAYNES
> > IT IS NOT A SCAM OR JUNK MAIL PLEASE READ!
> > Written by David Kanecki, MBA, ACS, Bio. Sci.
> > November 25, 2007
> > Fcs cytopro quick Facs 6.0 java 14
> > The FCS Cytopro Quick Facs 6.0 Java allows the performance of
> > flow
> > cytometry analysis on computers or PC. This new technology allows for
> > utilization on Mac, Linux,
> > Solaris, and Windows with the same functionality and processing
> > speed.
> > It is designed to minimize the learning curve by doing away with a
> > confusing menu bar.
> > This new easy navigation process allows for any level of student,
> > technologist or researcher to perform flow cytometry analysis with
> > ease. The utilization time can be used inside or outside of the
> > lab.
> > Easy operation allows for greater efficiency and quality. This
> > translates into decrease in time and cost.
> > About 27% overall savings in flow cytometry experiments. So,Just
> > pick, click and go!
> > This program can read three of the four most common modes of the FCS
> > 3.O files, and is
> > Backward compatible with FCS 2.O and 1.O files
> > Equipped with advanced processing speeds allows rapid results, no
> > need to wait for your computer to think. When performing a batch mode
> > analysis, you can analyze about 24 to 42,000 samples per hour with
> > FCS
> > 2.0 files, and about 4,000 samples per hour with 1 million event by 9
> > parameters for FCS 3.O files. Also 10 million event by 25 parameter
> > FCS 3.0 file or a 10 million event FCS 2.0 and 1.0 files.
> > This FCS Cytopro Quick Facs Java 6.O is for large-scale and rare-
> > event analysis.
> > There is no Dongle, License fees, or restrictions for using the
> > software. The user can process the analysis where ever and whenever
> > necessary.
> > There are various features this program has to offer consisting of
> > analyzing dot plots, dual histograms , and descriptive statistics.
> > Easy to use just copy and paste the items into your report or paper.
> > This shows the result of a du
> > al, histogram analysis along with dual, descriptive statistical
> > analysis. The first histogram is in red, and the second is in blue.
> > The first, descriptive
> > analysis gives the information for the red histogram, and the second
> > descriptive analysis,
> > below the first, gives the information for the blue histogram. Also,
> > in the result box, see the two files listed along with their
> > checksums. The checksum is a measure that allows you to monitor the
> > correctness of a file. If a file is the same as the last time, its'
> > checksum will be the same. If the file is different, same size, but
> > different data, then its' checksum will be different:
> > Conclusion
> > Without a dongle, license fees and excessive time wasted educating
> > to
> > on software usage, Kanecki Associates Inc will save the world
> > millions.
> > There will be no need for large seminars and wasted time on how to
> > utilize software and more time to study important issues. Students
> > can
> > learn without fear of being able to afford the software. For only one
> > hundred and fifty dollars USD. for students. Yes you can own it.
> > There
> > is no license fees.
> > If you are not a student the cost is only two hundred and fifty
> > dollars USD .So why lease your software from the lab at thirty
> > dollars
> > per hour? Within five hours of leasing software from you lab at 30
> > dollars per hour you could be at home relaxing and working on you PC.
> > If you are not a student the cost is only two hundred and fifty
> > dollars. Never a license fee or dongle. You can take it or leave it,
> > where ever you like!
> > The FCS Cytopro Java 6.0 program allows you to analyze large
> > sample
> > sets
> > quickly, easily, and with high-quality and precision. In addition,
> > it
> > can analyze large data
> > set files of up to 10 million events and 25 parameters for use in
> > rare-
> > event analysis or
> > kinetic based experiments. This program is designed so that it is
> > easy to use, and this ease
> > of use and accuracy translates into high productivity and cost
> > savings, about 27% overall
> > in flow cytometry cost.
> > New technology breaking down the walls developed by Dr. David
> > Kanecki,
> > No it is not a Scam or Junk mail! It is high tech software at a
> > reasonable price.
> > Free trial down load so there is nothing to loose
> > http://www.kanecki.comhttp://www.flowcytometrysoftware.com
> > direct download link for free trialKanecki Associates - The World Leader in Intelligent Thinking Systems Management Government Military Intellectual Property
> > FCS Cytopro Java 6.0 Script (c) 2007 By D. Kanecki, All Rights
> > Reserved
> > Mi...@Kanecki.com
> > WHAT PEOPLE SAY ON THE MAIL LIST ABOUT THE PRICE AND LICENSE FEES FOR
> > SOFTWARE!
> > FACS-software
> > * This message: [ Message body ] [ More options ]
> > * Related messages: [ Next message ] [ Previous message ] [ Next
> > in thread ] [ Replies ]
> > From: Yuecel, Raif <Raif.Yue...@pmintl.com>
> > Date: Wed Nov 02 2005 - 06:32:42 EST
> > Dear FACS-experts,
> > I need the opinion of the specialist:
> > I need FACS-software for PC and had decided for FlowJO. I know the
> > mac-version of FlowJO. Indeed, this is very expensive.
> > Now, I was asked to get a comparative offer. Therefore, I thought
> > about
> > WinList. This is much cheaper. But I have no experience with WinList.
> > My question to the experts:
> > 1. What are the advantages and disadvantages of both software?
> > Question of the business & finance department, which are also
> > entitled:
> > 1. Why is FlowJo so expensive, if you need several licence?
> > 2. Why FlowJo and not WinList, because WinList much cheaper?
> > Thanks very much for the help !
> > Best regards
> > Raif
> > -----------------------------
> > Dr. Raif Yuecel
> > PHILIP MORRIS Research Laboratories GmbH
> > Fuggerstrasse 3
> > D-51149 Koeln
> > Germany
> > Phone +49 2203 303 486
> > Fax +49 2203 303 362
> > =====================
> > This e-mail message is for the sole use of the intended recipient(s)
> > and may
> > contain confidential information. Any unauthorized review, use,
> > disclosure
> > or distribution is prohibited. If you are not the intended recipient,
> > please
> > contact the sender by reply e-mail and destroy all copies of the
> > original
> > message.
> > Diese e-mail ist ausschließlich für den/die Adressaten bestimmt und
> > kann
> > vertrauliche Informationen enthalten. Jede nicht genehmigte Nutzung,
> > Veröffentlichung oder Weitergabe ist untersagt. Sollten Sie nicht der
> > für
> > diese e-mail bestimmte Adressat sein, kontaktieren Sie bitte den
> > Absender
> > und vernichten alle Kopien der Original-Nachricht.
> > Received on Wed Nov 2 17:38:00 2005
> > * This message: [ Message body ]
> > * Next message: Robert C. Leif: "RE: FCS files and software
> > compatability"
> > * Previous message: Stingley, Robin L: "FACSAria service"
> > * Next in thread: David Novo: "Re: FACS-software"
> > * Reply: David Novo: "Re: FACS-software"
> > * Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
> > Subject ] [ By Author ] [ By messages with attachments ]
> > This archive was generated by hypermail 2.1.8 : Sat Jan 14 2006 -
> > 22:03:58 EST
> > *****************************************************************************-
> > ***********************************************
> > THIS REPLY FROM MITCHELL HAYNES KANECKI ASSOCIATES INC.
> > WHAT PURDUE DID NOT WANT YOU TO KNOW....
> > FCS CYTOPRO JAVA 6.0 FREE TRIAL DOWN LOAD
> > $150.00 STUDENTS
> > NO LICENSE FEES
> > FCS 3.0http://www.kanecki.com/flowF.htmlhttp://www.flowcytometrysoftware.com
> > ****************************************************************************************WHAT
> > DO THE STUDENTS HAVE TO DEAL WITH AND SAY?
> > Purdue University Cytometry Laboratories - Flow cytometry
> > confocal ...... please go to the rippoff website above and make your
> > complaint and register. ... J. Paul Robinson, Ph.D., Director PUCL,
> > 1203 W. State St., Purdue ...www.cyto.purdue.edu/flowcyt/rebates.htm?institutionId=694&meetingId=218
> > - 8k - Cached - Similar pages - Note this
> > STUDENT CONTRACT THROUGHT PURDUE FOR SOFTWARE THEY SHOULD HAVE
> > A LAWYER READ BEFORE THEY SIGN***POLARIS AGREEMENT
> > "POLARIS" SOFTWARE LICENSE AGREEMENT
> > (INCLUDING LIMITED NON-COMMERCIAL DISTRIBUTION RIGHTS)
> > UNIVERSITY OF ILLINOIS
> > Under this Agreement, The Board of Trustees of the
> > University
> > of Illinois ("University"), a body corporate and politic of the State
> > of Illinois with its principal offices at 506 South Wright Street,
> > Urbana, Illinois 61801, U.S.A., on behalf of its Department of
> > Computer Science on the Urbana-Champaign Campus, provides the
> > "POLARIS" software ("Software") described in Appendix A, attached
> > hereto and incorporated, to Purdue University ("Licensee") subject to
> > the following terms and conditions:
> > 1. The parties acknowledge that Licensee currently has a
> > license
> > that restricts its use of the Software to internal use, and Licensee
> > has requested the University to grant certain distribution and
> > sublicensing rights for the Software and derivative works thereof.
> > Accordingly, upon execution of this Agreement by Licensee below, the
> > University grants, and Licensee accepts, a royalty-free, non-
> > exclusive, non-transferable license:
> > A. To use, display, install, copy and distribute unlimited copies
> > of the Software for its own internal academic and research purposes.
> > B. To make derivative works of the Software. However, if
> > Licensee distributes any derivative work based on or derived from the
> > Software, then Licensee will (1) notify the University (c/o Professor
> > David Padua, e-mail: pa...@uiuc.edu) regarding its distribution of
> > the derivative work and provide a copy of the modified Software to
> > the
> > University, if requested by the University, and (2) clearly notify
> > users that such derivative work is a modified version and not the
> > original Software distributed by the University.
> > C. To redistribute (sublicense) the Software or derivative works
> > based on the Software, in whole or in part, to third parties provided
> > that (1) the University's copyright notice and any accompanying
> > legends or proprietary notices are reproduced on all copies that
> > include the University's original code, (2) no royalty is charged for
> > such copies, and (3) third parties are restricted to using the
> > Software for academic and research purposes only.
> > No license is granted herein that would permit Licensee or
> > its sublicensees to incorporate the Software into a commercial
> > product, or to otherwise commercially exploit the Software. Should
> > Licensee or its sublicensees wish to make commercial use of the
> > Software, such Licensee or sublicensee should contact the University,
> > c/o its Office of Technology Management (o...@uiuc.edu; Telephone
> > (217)333-7862) to negotiate an appropriate license for such
> > commercial
> > use.
> > 2. THE UNIVERSITY GIVES NO WARRANTIES, EITHER EXPRESSED OR
> > IMPLIED, FOR THE SOFTWARE AND/OR ASSOCIATED MATERIALS PROVIDED UNDER
> > THIS AGREEMENT, INCLUDING, WITHOUT LIMITATION, WARRANTY OF
> > MERCHANTABILITY AND WARRANTY OF FITNESS FOR A PARTICULAR PURPOSE.
> > Licensee understands the Software is a research tool for
> > which no warranties as to capabilities or accuracy are made, and
> > Licensee accepts the Software "as is¡±, without maintenance,
> > debugging, support or improvement. Licensee assumes the entire risk
> > as to the results and performance of the Software and/or associated
> > materials. Licensee agrees that University shall not be held liable
> > for any direct, indirect, consequential, or incidental damages with
> > respect to any claim by Licensee or any third party on account of or
> > arising from this Agreement or use of the Software and/or associated
> > materials.
> > 3. Licensee understands the Software is proprietary to the
> > University. Licensee will take all reasonable steps to insure that
> > the source code is protected and secured from unauthorized
> > disclosure,
> > use, or release and will treat it with at least the same level of
> > care
> > as Licensee would use to protect and secure its own proprietary
> > computer programs and/or information, but using no less than
> > reasonable care.
> > 4. In the event that Licensee shall be in default in the
> > performance of any material obligations under this Agreement, and if
> > the default has not been remedied within sixty (60) days after the
> > date of notice in writing of such default, University may terminate
> > this Agreement by written notice. In the event of termination,
> > Licensee shall promptly: (i) return to University all copies of
> > licensed Software and derivative works thereof in tangible or
> > electronic form that are in Licensee's possession or control; or (ii)
> > permanently destroy or disable all copies of the Software in
> > Licensee's possession or control, except as specifically permitted by
> > the University in writing; and (iii) provide University with a
> > written
> > statement certifying that Licensee has complied with the foregoing
> > obligations. All rights and licenses granted to Licensee shall
> > terminate upon such termination. In the event of any termination of
> > this Agreement, any and all sublicenses granted by Licensee to third
> > parties pursuant to this Agreement (as permitted by this Agreement)
> > prior to the date of such termination shall nevertheless remain in
> > full force and effect.
> > 5. In all uses of the Software, where appropriate, Licensee will
> > credit the origins of the Software, or derivative works based
> > thereon,
> > to the creator(s) and the University of Illinois for their role in
> > the
> > development of the Software.
> > 6. This Agreement shall be construed and interpreted in
> > accordance with the laws of the State of Illinois, U.S.A..
> > 7. This Agreement shall be subject to all United States
> > Government laws and regulations now and hereafter applicable to the
> > subject matter of this Agreement, including specifically the Export
> > Law provisions of the Departments of Commerce and State. Licensee
> > will not export or re-export the Software without the appropriate
> > United States or foreign government license.
> > Appendix A: Software Identification
> > Form Approved by University Counsel, M.A.R., _1/5/98_
> > USER DIRECTORIES:vcr:stipswor:Software Lic:distlpol
> > APPENDIX A
> > to
> > "POLARIS" SOFTWARE LICENSE AGREEMENT
> > (INCLUDING LIMITED NON-COMMERCIAL DISTRIBUTION RIGHTS)
> > UNIVERSITY OF ILLINOIS
> > Identification of Licensed Software
> > Title: POLARIS
> > Version Number: All versions provided to Licensee under license
> > from
> > the University
> > Creator(s): David Padua, Jay Hoeflinger, Paul Petersen, Rudolf
> > Eigenmann, William Blume, Keith Faigin, Peng Tu, and Steven
> > Weatherford
> > Responsible Unit(s): Department of Computer Science (formerly
> > Center
> > for Supercomputing Research and Development)
> > Description : Polaris is a source-to-source Fortran restructurer. It
> > parses Fortran programs and represents them internally using a
> > hierarchy of classes. Polaris contains analysis modules to identify
> > dependences, private data, reductions, and induction variables. It
> > also contains a code generation phase that targets parallel Fortran
> > extensions used by shared-memory machines.
 
Purdue Cytometry Mail List ..US ISAC members Please write your Senator for $7 BILLION

:clap2:.....J PAUL ROBINSON :drum:

US ISAC members - Please write your senator
This message: [ Message body ] [ More options ]
Related messages: [ Next message ] [ Previous message ]
From: Professor J.Paul Robinson <j...@flowcyt.cyto.purdue.edu>
Date: Sat Jun 10 2006 - 10:55:20 EDT

[ Coordinator's note:
[ The URL in this message links to a FASEB page
[
[ Steve :bolt::sorry:

Colleagues: If you are in the USA, please consider sending your
senators the letter you
will find at this FASEB site. Senators do take note of these emails
and letters - this is
a $7 billion addition to the budget. It is vital that we maintain NIH
funding - Many ISAC members
are dependent upon quality reseach funding. :typing:

J. Paul Robinson
President, ISAC :tea:

Click on this URL to take action now
FASEB
If your email program does not recognize the URL as a link,
copy the entire URL and paste it into your Web browser. :nerd:


:laser::dance:
 
NIH Increase

:whip:> ***************************************************************************­­*****************************************************
> June 29, 2007 :peace:
> NIH Funding
> Another thread from Purdue worth posting:

:hat::hat::hat::hat::hat::hat::hat::hat::hat::hat::hat::hat::hat::hat::hat::hat::hat::hat:

> We are calling upon you again during this Independence Day holiday
> period to ask you to contact your Representative in person at a local
> event or by telephone to the local office by July 9th. When Congress
> returns to Washington after the 4th of July recess, the House of
> Representatives will consider a 1.9% increase to the NIH budget.
> Between now and then, you have the opportunity to remind your
> Representative of the importance of your work and let them know about
> any difficulties you have encountered because of shrinking budgets.

> Members of Congress have told us that they are not hearing from
> researchers in the community. We must turn this around. Members and
> their staffs are receptive to hearing from researchers about how the
> proposed FY 2008 budget will affect their research and their careers.

> Please contact your Representative either in person at a local
> Independence Day event or by telephone before July 9th. Tell them
> your
> story and let them know that they must make cancer and biomedical
> research funding a national priority.
:ranger::ranger::cheer2::cheer2:
> In your contact with your Representative, please mention any of the
> research challenges you are facing at this time, for example: -
> denials
> or delays in getting grant funding for meritorious research
> -the impact of cuts to grants already funded
> -staffing and equipment shortages
> -the loss of bright young investigators to other fields
> -reduced opportunities for patients to get on therapeutic clinical
> trials
> -cancellation or halting of important clinical trials.

> In short, your Representative needs to hear about the serious effect
> these low budgets are having on your research progress. Urge them to
> make cancer research funding a national priority. A few minutes of
> your time could help get NIH and NCI funding back on track.

> Thank you for responding to this request and have an enjoyable
> holiday.

> William N. Hait, M.D., Ph.D., President
> ***************************************************************************­­****************************************************
> William G. Nelson V, M.D., Ph.D., Chair, Science Policy & Legislative
> Affairs Committee

> AACR Science Policy Alert: 1.9 Percent Increase to the NIH Budget
> William G. Nelson V, M.D., Ph.D., Chair, Science Policy & Legislative
> Affairs Committee. Margaret Foti, Ph.D., M.D. (h.c.), Chief Executive
> Officer ...www.aacr.org/page10907.aspx- 24k - Cached - Similar pages - Note this

> MUST READ!

> ***************************************************************************­­****************************************************
> Margaret Foti, Ph.D., M.D. (h.c.), Chief Executive Officer
> P.S. Please let AACR know you have made a contact with your
> Representative by sending us a quick e-mail at legaffa...@aacr.org.
> Just write: "I have contacted Representative X in person/by phone
 
Purdue Cytometry Mail List Coalition for Cytometry Worldwide ISAC CONGRESS

Vote for the Coalition for Cytometry Worldwide :spider:

This message: [ Message body ] [ More options ]
Related messages: [ Next message ] [ Previous message ]
From: Henning Ulrich, Ph.D. Henn[URL="http://groups.google.as/groups/unlock?msg=4b14a1c1be96e8a8&_done=/group/misc.health.aids/browse_thread/thread/4564c83c337c8210"]...[/URL]@iq.usp.br :sick:

Date: Tue Mar 25 2008 - 13:33:43 EDT :grouphug:

Dear ISAC Member, :mod:


For the upcoming elections of new members of the :director::hurt:
ISAC council I would like to ask you to vote for
our "Coalition for Cytometry Worldwide".

ISAC is a mature and highly recognized scientific society
with a strong history of supporting research,
education, and technological innovation. :scared::puke:

The members of this coalition will work to have ISAC
continue to prosper and expand its visibility
into the areas of the globe that are still underrepresented in our
society.
:violin:


All members of the Coalition for Cytometry
Worldwide are internationally recognized highly
active scientists. :mad::)

They will continue to support maintenance of our high standards in science, technology and education and will continue to
bring cytometry forward.:laser::rolleyes:

All members have served for the society during their career in different
areas and will maintain ISAC's present high standards in new regions.

In order to support our task of bringing ISAC and
Cytometry forward we would encourage you to vote
for the list of candidates below. :spider:

Please invite also your colleagues and friends to vote for our list.
The deadline date for voting is Wednesday, March 26, 2008.

Coalition for Cytometry Worldwide :emot0:


President Elect: Paul Smith (E) :eek:

Secretary (1): John Nolan (US) :emot1:



Biological Councilor (3):

Patrice Petit (E);

Henning Ulrich (SA):eek:


Clinical Councilor (2). Shazib Pervaiz (A)

Markus Barten (E)


Technical Councilor :jaw: (1). Robert Leif (US)


Thank you!

Kind regards,
Henning Ulrich, Ph.D.
Associate Editor Cytometry Part A
-------------------------------------------
Associate Professor
Departamento de Bioquimica
Instituto de Quimica
Universidade de Sao Paulo
Sco Paulo - 05508-900
Brazil
E-mail: henn...@iq.usp.br
Alexander Henning Ulrich
Tel. +55-11-3091-3810
Fax. +55-11-3815-5579
Received on Wed Mar 26 15:38:00 2008
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Next message: Irene Hatzinisiriou:

"web based calendars (ON-LINE
SCHEDULING SOFTWARE)"


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Purdue Cytometry Mail List Flow Jo and Verity Software

RE: DNA analysis software
:laser::crutch::p

* This message: [ Message body ] [ More options ]
* Related messages: [ Next message ] [ Previous message ]
[ Maybe in
reply to ] [ Next in thread ] [ Replies ]

From: Turbov, Jane <JTur...@enh.org> :sorry:
Date: Wed May 23 2007 - 17:33:27 EDT
Hello Flow-ers, :brick::faint:

I would like to revisit the question of DNA analysis. We have ~ 12
year
:ballchain::whip:
old FacsCalibur and have just upgraded our system to Mac Os X.

We had

ModFit LT 2.0 installed previously. :bathbaby:

We are having problems installing

:smash::smash:
Mac Classic but hopefully this will be resolved soon.


From the Verity website, it appears that ModFit 3.0 can run off of Mac OS X but printing may be an issue.:doh::faint:

http://www.vsh.com/Support/KBDetail.asp?incident=1132

Our lab manager suggested ;):) FLOJO


http://www.flowjo.com/specproliferation.html:emot2:


but


this might be too sophisticated for our needs.:bathbaby::amen:

Does anyone have any recommendations?

Thanks,

Jane Turbov
Department of :bathbaby:OBGYN Research


ENH Research Institute

2650 Ridge Avenue

Evanston, IL 60201

TEL: 847-570-4021

FAX: 847-733-5256

_____
From: VSH Tech Support [mailto:T...@vsh.com]
Sent: Wednesday, February 21, 2007 10:30 AM
To: cyto-inbox

Subject: RE: DNA analysis software
Hello Ibtissam,
ModFit LT, for PC or Mac,

has advanced modeling capability for
research applications

in

DNA cell cycle analysis.

You may use any of the model templates the program offers, or create your own models for
non-traditional analysis, including non-mammalian DNA cell cycle
studies.

ModFit LT can be linked to our WinList program

to provide a complete cell cycle analysis on any number of sub-populations with a

single click of a button. For an overview, visit http://www.vsh.com/products
:tea:

<http://www.vsh.com/products> .
Best regards,

Don

Donald J. Herbert


Technical Support Manager
Verity Software House

________________________________
 
Re: Last words from Mario (we hope) on data display

Re: Last words from Mario (we hope) on data display

:drum:

From: Howard Shapiro (h...@shapirolab.com)
Date: Sat Oct 04 1997 - 22:16:13 EST


* Next message: BI...@Darwin.Stanford.EDU: "Re: data display
(contours,etc)"
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I will propose to Cytometry to write a perspective on the topic of FACS data display. :deadhorse:


>I challenge any of those of you who
:fencing:

>champion dot plots (or even color dot plots) to join my effort and write a
>"counterpoint" analysis to provide a balancing viewpoint.


A forum with various viewpoints, well documented and illustrated,
would probably be very helpful to the readership.


:bolt:

>Calman writes about dot plots:

>> Furthermore it stresses the single cell nature of the
data- each dot is a cell.

No, no, no, no, no, no, no! :frusty::humble::target:


This is precisely the problem!

In dot plots, each
dot can be one cell, two cells, three cells, or a thousand cells. You can
never
know which. :scared:



I think what he probably meant was that a dot plot does allow you to
spot
numbers of occurrences below your lowest threshold value for
contouring,
including single occurrences; it is true that if there isn't a dot at
a
point on a dot plot there weren't any cells observed :bathbaby::photo:

with the corresponding data values.



I agree with Alice that the precise way of contouring can affect how the more frequent populations appear.

This is why the choice of contouring algorithms is so important!

One of the most robust (in that it is objective, not allowing
"user-defined" contouring levels, etc.) is probability contouring. This method
of contouring has been adopted by SAS Institute for use in their bivariate
displays--in addition, it is offered by several FACS data analysis packages.


>This method of contouring generates displays that are indepedent of the number of events collected --

something that no other display can do. :boxing:


By "probability contouring" do you mean normalization, so the contour
lines
represent percentile values rather than absolute numbers of cells?


This is a very sensible method of displaying things, and facilitates
comparison of samples of unequal sizes.

In order to deal with rare events, however, you
still have to have dots or their equivalent added to the contour plot.

:juggle:
Thus, using Dot plots or color dot plots or user-defined thresholding,
I can make a variety of
>conclusions about the same sample depending solely on how many events I choose
>to collect (or display)!

>Jim Houston is 100% correct that the precise method :bathbaby:of data display is critical. :amen:

>I urge reviewers and editors to demand that this information be included
in all
>FACS data displays.


Note, for example, that bivariate chromosome contour plots are
generally made with higher thresholds than plots of immunofluorescence...

and
it wouldn't be a bad idea to include a scale or to indicate which contour
lines represent which percentiles.


Once again, this brings us to the fundamental point of data display: to convey information accurately to the reader.

I highly recommend a book by Edward Tufte, "The Visual Display of Quantitative Information," about this topic (especially to programmers developing analysis packages).

This fabulous book

>shows how misleading different styles of graphs can be, and discusses some of
>the underlying principles of data display--principles largely ignored by
>developers of FACS data analysis programs.
>There was some discussion about art vs. science. Do not mistake artistry for
>disinformation! Of course there is art in science, and in the presentation of
>scientific data.


If not, we would only see tables of numbers that would be
>incomprehensible-- :jaw: we are, after all, only human.



Tufte actually has three books out; each is a work of art as well as a
work of science.:hail:

In the Chapter on Data Analysis in the 3rd Edition of
Practical Flow Cytometry, I suggest that single parameter distributions be
represented using Tufte's minimalist version of the "Box and Whiskers" plot, which
shows the position of the median, 25th and 75th, and 5th and 95th percentile
values (or, alternatively, the full range of the data instead of 5th
and 95th %).

This could readly be extended to a two-dimensional version,
but
might be better represented by different colored (or differently
shaded)
areas than by contour lines.


-Howard

* Next message: BI...@Darwin.Stanford.EDU: "Re: data display
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RE: data display

From: David L. Haviland, Ph.D. (dhavi...@imm2.imm.uth.tmc.edu)
Date: Fri Oct 03 1997 - 15:31:37 EST

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Greetings all:

I have learned a great deal and have enjoyed the discussion concerning
data display, dot vs contour, etc.

To settle a curiosity that I had, :bathbaby:

I privately asked M_Roederer this question. :gossip:

"We know each plot (contour/dot/density) has its benefits and
pitfalls.
However, does anyone know of an instance where a conclusion or part
thereof
has been in serious doubt because a conclusion was drawn off a dot
plot,
when a contour plot would have suggested a different conclusion?"

Frankly, I expected "no" as the respone but was suprised when Mario
stated
there had been a few instances. So my question to the group is

does anyone have such a reference(s) of a flow cytometric "opps"? Were manuscript retractions involved?

Many thanks in advance,
David

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RE: data display

From: Donnenberg, Albert (donnenber...@MSX.UPMC.EDU)
Date: Tue Sep 30 1997 - 15:41:23 EST
* Next message: Ray Hicks: "Re: Last words from Mario (we hope) on
data display"
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Dot plot or probability plots, what do you do when compensation forces
a significant number of events into the first channel? Dot plots don't
get any blacker than black, some contour algorithms truncate the first
channel of data!

In the 4-color world it is sometimes impossible to keep events out of
the gutter when PMTs are balanced and compensation is optimized.
Albert D. Donnenberg, Ph.D.



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RE: data display

From: Gib Otten (G...@cellgenesys.com)
Date: Tue Sep 30 1997 - 19:32:44 EST

* Next message: Michael Feldhaus: "Position available"
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Somtheing that's been overlooked in this discussion.

While a dot plot by itself may be misleading because of the dot density problem, most
investigators identify regions of interest and indicate the percent cells in those regions. The same should be done, and probably is being done, with contour plots.


Gib Otten, Ph.D.
Senior Scientist, Gene Therapy Applications
Cell Genesys, Inc.
342 Lakeside Drive
Foster City, CA 94404
Tel: (415) 425-4515
email: g...@mail.cellgenesys.com
> ----------
> From: Alice.L.Gi...@dartmouth.edu
> Sent: Monday, September 29, 1997 9:48 AM
> To: Cytometry Mailing List
> Subject: re: data display

> I know that dot plots can be misleading for all the reasons that

Mario Roederer describes --- :bathbaby:

BUT I also know that, by choice of the contouring
algorithm, you can make a contour plot look any way you want: :doh:shoulders on peaks

can be emphasized or can be made to disappear, double peaks can be made to look like single peaks, etc etc etc.. These problems are not solved by showing the dots that are below the contouring threshold, as they relate to the levels of coutours above the threshold.

Dot plots can be misleading, but contour plots are a can of worms.

OK Mario (and anyone else) :hug: -- looking forward to your response!

> Alice :nono::bathbaby:

> Alice L. Givan
> Englert Cell Analysis Laboratory
> Dartmouth Medical School
> Lebanon, New Hampshire
> NH 03756 USA
> tel 603-650-7661
> fax 603-650-6130
> e-mail gi...@dartmouth.edu


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Purdue Cytometry Mail List BD's Fix pseudo-unique ID's in their infinite wisdom

From: David.C.McFarl[URL="http://groups.google.fm/groups/unlock?msg=d45469e2d6372c2d&_done=/group/misc.health.aids/browse_thread/thread/01c6222c93779c6d/d45469e2d6372c2d%3Flnk%3Draot"]...[/URL]@gsk.com

Sent: Wednesday, July 19, 2006 9:06 AM
To: Cytometry Mailing List

Subject: Re: FloJo and DiVa file IDs :mad:
:couch2:
Ray, :eek:

I had this discussion with BD a while ago. :emot1:This is something that
blindsided me. :3_8_14[1]:

We actually have an SOP that we follow for tube IDs
such that they are all unique. Basically, we use accession numbers
of
the format FCM00001 for experiment names
and then the tube IDs would be FCM00001_001, etc.

In the older versions of Diva, this was the "FCS" file name that showed up in the folder when experiments were exported.
:croc:

However, those weren't "real" FCS files.

Evidently,

people were trying to import these bogus FCS files

back into Diva,:violin:

or

elsewhere,

and it was creating errors.

In their infinite wisdom,

BD's fix :bathbaby:

was

to change the names of these files to something unrecognizable to the users so that they wouldn't be tempted to do that.

They have a system of generating their own pseudo-unique IDs in this manner.:spider:

I say pseudo-unique,:( because it isn't clear to me where/how the
numbering starts if, for instance, you have several instruments
running Diva in the same lab, or if upgrade to a new version by doing
a clean install.

Or perhaps the machine crashes. :noidea:

Can the numbering be set to pick up where if left off?

I think what they really mean is unique to that particular incarnation of the database on that local machine which isn't unique enough in my mind.
:3_8_13[1]:
This really screwed up my system.

So asked the same question you just did, "Is there a way
to retain my Tube IDs

and/or

can I configure Diva to use the same unique filenaming convention that I do".

Of course, the answer was :jaw: no.

Even worse,

even if you export as FCS files, although recognizable, the files aren't exactly the same as the entered ID.

The specimen name :whip:

and

an underscore are added before the tube ID

and

".fcs" is added to the end.

This is more aggravation for me to deal with in regard to data management in a GLP setting.

It's too bad. :mad:We like exporting entire experiments for archiving purposes,

but

it is easier to reconcile file name/tube ID discrepancies when exported as
FCS, so we will probably have to make a change.

At present we've just been adding a file note to the archive documents and explaining that the original naming scheme will reappear when imported back into
Diva.

This isn't entirely true when FCS files are imported into Diva
since you have to import them into an open experiment, which could be
named differently than the exported FCS files.



Again this is an issue for me in a GLP validated instrument setting and would warrant more workarounds or further explanation as to why reimported raw data
doesn't look "exactly" as it did when collected.

Let me know if you here anything different from what I've told you as
the situation seems to change frequently and this may no be the most
current info.

Regards,

Dave

David McFarland
Principal Scientist ;)
GlaxoSmithKline


"Ray Hester" <rhes...@jaguar1.usouthal.edu>
17-Jul-2006 16:16

To "Cytometry Mailing List"
<cytome...@flowcyt.cyto.purdue.edu>
cc

Subject FloJo and DiVa file IDs :eek:

Can someone tell us how to export DiVa files as 'Experiments', not as
'FCS files', so that the individual tubes within the 'Experiment'
retain
their specific identities, e.g., 'T4/8', rather than some numerical
value, e.g., '2437'. When we export DiVa data as 'FCS files', the
specific tube ID is retained.

We have a FloJo 7.1 USB dongle :target:

and it's being used primarily on PCs.

Thanks.

Ray Hester
Univ. of South Alabama


I must say that it came as a complete surprise to me that

BD

has not notified all Aria-containing laboratories about this bug :director:

(I was visiting some other laboratories last week, and this news came as a
complete surprise to them! :brick::faint:

The laboratories were relieved to find out that the recent sorting failures
were not their fault). :emot0:

Normally, just in the last two weeks, well after the problem was identified.

Henc BD :argue:
has been responsive to problems,

but

in this case they have completely dropped the ball.


:fish:


Diva 5.0 Software on Aria

This message: [ Message body ] [ More options ]
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thread ] [ Replies ]

From: Mario Roederer roede[URL="http://groups.google.fm/groups/unlock?msg=d45469e2d6372c2d&_done=/group/misc.health.aids/browse_thread/thread/01c6222c93779c6d/d45469e2d6372c2d%3Flnk%3Draot"]...[/URL]@drmr.com

Date: Tue Oct 10 2006 - 08:37:47 EDT

Dear Aria Users:

We would like to alert you to a serious bug in the Diva 5.0 software
that applies only to sorting on the Aria.

There is a significant

bug

that causes misapplication of gates, such that your sorting is completely incorrect.

We notified BD about this

bug:3_9_7[1]:

on on September15,

and

they were able to reproduce it before the end of September.

The problem occurs on any sorting that requires new gates; sorting
using old templates seems to work in at least some cases.

We have taken the solution to go back to the previous version of

Diva software (4.1.2) until BD can fix the issue.

Note that we have seen no issues with Diva 5.0 software on our LSR II analyzers,

and

continue to use 5.0 on the LSR II.

I must say that it came as a complete surprise to me that BD has not
notified all Aria-containing laboratories about this bug :eek:

(I was visiting some other laboratories last week, and this news came as a
complete surprise to them! The laboratories were relieved to find
out that the recent sorting failures were not their fault).

Normally, just in the last two weeks, well after the problem was identified. HencBD
has been responsive to problems,

but

in this case they have completely dropped the ball.

It cost us two important experiments to discover the bug;

in visiting other laboratories, I discovered those laboratories lost several important experiments e,

I felt it necessary to let everyone know before more valuable
experiments are lost.

mr :emot1:

Received on Tue Oct 10 10:58:00 2006
This message: [ Message body ]
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Previous message: Stevan Lauriault: "Isotype Control Structure"
Next in thread: David.C.McFarl...@gsk.com: "Fw: Diva 5.0 Software on
Aria"

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Aria"
Maybe reply: Matt Wikstrom: "Re: Diva 5.0 Software on Aria"
Maybe reply: Haviland, David L: "RE: Diva 5.0 Software on Aria"
Maybe reply: Rice, Susan E. \(Fka Grigsby\): "FW: Diva 5.0 Software
on
Aria"

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Purdue Cytometry Mail List FC500 data Files a COMPLICATED BEAST!

From: Novo, David [email protected]
:mad:
Date: Fri Aug 17 2007 - 18:52:53 EDT


Hello Kate,
:emot2:

The FC 500 data files are indeed a complicated beast that give you a
lot of information.
:3_8_14[1]:

There are indeed two separate data sets inside the one
FC500 file.

This is allowed by the FCS standard.
:eek:

The first data set is a FCS 2.0 file.

quite a typical FCS 2.0 file, stored in 10 bit with the data already saved compensated and This is converted to log (if necessary). :3_9_7[1]:

This is quite clever to put it there because any analysis software that can read FCS 2.0 files should be able to read this file, and not even know that there was a second data set hiding afterwards.

I am not sure why the BD CBA software:emot1:

would not be able to handle this,

maybe there is a hidden setting or something that allows this.
:3_8_13[1]:

The second data set is a high resolution data set that is in FCS 3.0
format. This contains all the high resolution linear data, that is stored uncompensated, and is ideal for performing software compensation.
:confused:

I guess our web site is either too high, or too low,

and :bathbaby:

fell out of the range of your previous search :) :)

FCS Express can read both the FCS

2.0 and FCS 3.0 portion of the FCS 500 data files. You can even export
them as individual files if you like. :attention:

When loading the files you can either default to load the FCS 2.0 or FCS 3.0 portion, or be prompted which to choose.


:jaw::behindsofa:
I also know that WinList can read both portions of the FC500 file.

I am not familiar with:laser::hail::bolt:FlowJo's :spider:capabilities in this regard.

If you contact them,:ban:

I am sure they will be glad to answer this point.


-Dave :pound:


-----------------------------------


David Novo
:hurt::peep:

President


De Novo Software


[email protected]
 
Purdue Cytometry Mail List BAN OF COMMERCIAL MARKETING Mario Roederer

Advertising:mad:

From: Mario Roederer ([email protected]):emot0:

Date: Fri Nov 01 2002 - 16:46:47 EST

:3_9_7[1]:
* Next message: William Telford: "Re: Fluorochromes choice"
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* Next in thread: Penney Robbins: "RE: Advertising"
* Maybe reply: Penney Robbins: "RE: Advertising"
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[ author ]
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________________________________________

I agree that there have been far too many commercial-oriented emails
on the list.:3_8_14[1]:


I appreciate the efforts of most manufacturers to
withhold from advertising on this list :hurt:

(plus, of course, the efforts of Steve & Paul to filter those out).:deadhorse::doh:

The list, which is an outstanding forum for exchange of information,
has been occasionally used to identify substantially new products:washing::eek:

which can significantly impact on how we do experiments.

I feel that the:sorry: :bolt:Molecular Probes email of 10/30

clearly does not fall into this category;

the new product advertised was no more than a slight
modification:3_8_13[1]: of the existing one.

Such an email should be directed solely to the person requesting information;:mad:

if that person then collates responses and puts it back on the list :hail: then so be it.

But

for manufacturers to directly respond in this way is

counter-productive to the goals of this list.:kev:


I would like to propose a 6-month moratorium on all emails that are
no more than advertisements.

Note that I write "would like to"--because I'm not sure that this is possible.:violin:

I don't want to put any additional on us on :tea:Steve or Paul to:painkiller: filter out the borderline emails.


While these may be easy to identify when they come from
manufacturers,
it could just as well be considered blatant advertising when they come from a user.:ban:


Therefore, perhaps we can see if the commercial participants on this
list could exercise self-restraint rather than requesting a formal
censorship of advertising emails.:lalala:


Thus, if you are a manufacturer, and you are responding to somebody's
request for information, :mad:do so privately to that person ONLY.

It is up to the person requesting information to decide whether or not the
information received in response to the query warrants a summary on
the list.


If you are not a manufacturer, and are responding to somebody's
request for specific information, please consider whether your
response
(that identifies a specific product or manufacturer)

is of general enough interest to warrant the list.

If it does not, then simply send it privately to the person who requested information, and let them decide whether to post the summary of responses.


In general,

I urge people to err on the side of caution and send
their information only to the person who requested it.

Realize that
if several people want the same information, they can always request
it from the original poster!

I have posted queries to the list;

people :kiss:have sent me emails asking me to forward to them the
responses, which:emot0:I did.


This process can significantly cut down on emails that might be
viewed as too commercial.:jaw::bathbaby:


mr
________________________________________
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[ attachment ]
________________________________________
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17:42:08 EST
 
Purdue Cytometry Mail List Im Developing an ANALYSIS program Called FCSPress

From: Ray Hicks (rh[URL="http://groups.google.com/groups/unlock?msg=762df1258bbd9f81&_done=/group/misc.health.aids/msg/762df1258bbd9f81"]...[/URL]@cus.cam.ac.uk)

> Date: Fri Jul 04 1997 - 04:15:27 EST

> Next message: Woo, Gary: "BrdU staining"
> Previous message: W. Roy Overton, Ph.D.: "RE: registration form"
> In reply to: Vincent Falco: "Software"
> Next in thread: Kevin G Waddick: "Fixation/Ab-binding"
> Reply: Kevin G Waddick: "Fixation/Ab-binding"
> Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
> [ attachment ]
> ---------------------------------------------------------------------------­­-----
> Hi Vincent, :3_3_21[1]:

> I'm developing an analysis program, based on FCS Assistant, called > FCSPress. :3_9_7[1]:

So far it's quite rudimentary and only allows you to plot
histograms.:eek:

It doesn't do gating or analysis yet. ;)

And

there is little support for the edit menu functions (cut, copy, paste, undo).

Unlike most cytometry programs available on the mac,

it allows you to open a data file and optionally manipulate its contents,

graphs are plotted from the active data window into graph windows,
which are wysiwyg for printing and saving as PICT )(sort of like cricket graph). :D

You can put as many graphs from as many files as you like into a graph window, and you can constrain their plotting to an optional grid of variable size.

You can annotate the graphs manually in variable fonts and styles, as well as semi-:pautomatically from text held in the source file.

I've got quite a way to go before the program is finished, but it seems to be quite stable so far.

If you'd like a copy let me know and I'll e- mail it to you (or anyone else who'd like a copy).

Ray

At 10:29 am +0000 3/7/97,

Vincent Falco wrote:

I have BD Vantage generated list files copied to a PC via FACSNET.:3_3_21[1]:

I have an investigator :emot1:who wishes to

generate multiple histograms in gallery format.

Which software product can do this the easiest(least steep learning curve)?

Thanks.

Ray Hicks


________________________________________________________________________
> |University of Cambridge |Tel 01223
> 330149 |
> |Department of Medicine |Fax 01223
> 336846 |
> |Level 5, Addenbrookes Hospital |e-mail
> <rh...@cus.cam.ac.uk> |
> |Hills Road Cambridge |Web http://facsmac.med.cam.ac.uk
> |
> |CB2 |ftp server ftp://131.111.80.78
> |
> |UK
> | |
> |_________________________________|
> _____________________________________|
> ---------------------------------------------------------------------------­­-----
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> [ attachment ]
 
Purdue Cytometry Mail List Software Marketing & Seminars!

FCSPress Training Sessions?

From: Ray Hicks ([email protected]):mad:

Date: Thu Oct 11 2001 - 19:42:36 EST
Next message: Laird Bloom: "RE: Multiple plate loader?"
Previous message: flow: "RE: HELP! How to clean the sample injection
tube?"
In reply to: Jennifer Wilshire: "FlowJo Training Sessions (Midwest)":emot0:

Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
--------------------------------------------------------------------------------
Most of you will findFCSPress so easy to use that you won't need:eek:
training sessions.

You can download a copy to try it out for yourselves at:emot1:

http://www.fcspress.com/html/FCSPArea.html

(Who's computer is this?)

where you'll also find documentation and details of a special offer.
If you find you DO need some assistance using FCSPress, :3_8_13[1]:
I'm planning on staying in Cambridge for quite some time, and I'd be happy to answerany ofyour queries via e-mail at

[email protected]
Cheers,:mad:
Ray
--
e-mail mailto:[email protected]
Web http://www.FCSPress.com
(Who's computer is this?)
Tel +44 797 453 8647
+44 1223 871081
Fax +44 870 7408595
--------------------------------------------------------------------------------
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tube?"
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--------------------------------------------------------------------------------
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17:40:27 EST

FlowJo Training Sessions (Midwest)

From: Jennifer Wilshire ([email protected])

Date: Thu Oct 11 2001 - 06:20:11 EST
Next message: Richard Grenfell: "Multiple plate loader?"
Previous message: William King: "Searching for pig anti-CD34"


Next in thread: Ray Hicks: "FCSPress Training Sessions?"

Rand locations are listed below. Thanks, Jennifer Wilshire FLOWJO
SEMINARS ______________________________________ Monday, October 15th
University Hospitals Clinical Sciences Center 600 Highland Ave,

Madison WI Room K4/418 10:00 AM reply: Ray Hicks: "FCSPress Training Sessions?"

Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
I will be in Wisconsin, Minnesota, and Iowa offering FlowJo seminars:emot2:
and training sessions next week. All of the hosting sites have
:confused::mad::emot0::emot2::p
graciously opened the sessions to members of the Cytometry list.


Times:3_8_14[1]:

______________________________________
Tuesday, October 16th University of Minnesota Cancer Center 425 E.
River Road Room 450 9:00 AM ¬ Introduction to FlowJo 10:30 AM ¬
Advanced Topics _______________________________________ Tuesday,
October 16th University of Minnesota, St. Paul Building AS/VM Room:(
295M 2:00 PM _______________________________________ Friday, October
19th University of Iowa South East 310 General Hospital Bean
Conference Room 10:00 AM _______________________________________ If
you are interested in hosting a FlowJo seminar, please let me know.
Upcoming trips are in the planning stages for: -NJ, PA, DC -Florida.
-
Boston (home) Jennifer Wilshire, Ph.D. Application Scientist:)
[email protected] Tree Star, Inc. FlowJo :3_3_21[1]:Home
Next message: Richard Grenfell: "Multiple plate loader?"
Previous message: William King: "Searching for pig anti-CD34"
Next in thread: Ray Hicks: "FCSPress Training Sessions?"
Reply: Ray Hicks: "FCSPress Training Sessions?"
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:mad:
 
Isac President J Paul & Purdue Cytometry Mail List End of Year Message

:emot0:From: J. Paul Robinson <j..._at_flowcyt.cyto.purdue.edu>

Date: Fri Dec 28 2007 - 13:43:46 EST


> Beware, the end is nigh! :mad:

No, not an apocalyptic prediction - but 2007 is definitely coming to an end. :clock:

Not before time I would say - it s been a busy year. :p

But

I have some strong words to end the year

and

I am going to say them!! :pound:

Of course you don t have to read them!
:behindsofa::brick:

Cytometry is now 40 years old and it s been sort of decaying a bit.


What do I mean?:eek:

I am amazed at how conservative

and

frankly boring the field has become.

Why?

It s time to move to the 21st century folks.

I'm getting older and frankly,

its time to kick some butt

as my younger colleagues often say.

We talk so much like it is the same old cytometry
it has always been.


Wake up people - times are changing

- look at all these new small companies
:spider: :spider:

trying to stick their noses in "our" field!
:mad: :target: :jaw:

True we need to do the core work and do it well,

but lets not forget that fundamental tools of cell analysis are changing

and

if we don't keep ourselves up-to-date and educated on what's happening....

before we know it, :bathbaby:

a new field will emerge and we will be like the old electron microscopists who are still wondering what happened ......


I know most of us work in the field :rant:

and

like what we do, :blah:

but

I think its time to open up a little

and

try to do some serious integration of our field.

It s not happening very effectively on the most part

I would say.:fish:


Cytometry is about integration of the tools of the field into the vast reaches of biological problems that we can contribute to solving.

Cytometry is about advancement of the field,
that means always looking ahead.

ISAC will soon be the International Society for Advancement

of Cytometry a 21st century Society not a 20th century Society.

Cytometry is not flow cytometry!!:yield:

Let s not kid ourselves about this folks.

Cytometry is about measuring cells -

however you do it - :humble:

and

flow cytometry is just one component of many.

I understand that it may be the only tool some of you use - :deadhorse:

I don t want to take away from that

or

de- emphasize its value or importance.

But,

we so often hear people talk about our field

only in context of just flow cytometry.

Recently, when we polled the ISAC community on

changing

our name from "analytical Cytology"

to Advancement of Cytometry"

we received comments like

"hey I don t do flow cytometry, :(

so

why are you reducing the breadth of the field?"

Ouch - they think "cytometry" means "flow cytometry".

We have a long way to go before


we convince the community that we cover all

aspects of cytometry.


And

let s also remember the growing membership

in

India and China :hug:

(that s half the worlds population right there)

it s high time we paid much more attention to these countries

as a field Awtar Krishan can t be the only person to drive cytometry training

and

education for 1.2 billion Indians can he?

Well he has been up to now.

Who is taking on the mantle of training and education of cytometry in
China?

So here's the scoop.


That's one of the reasons

why the Purdue Web portal is going to change. :3_8_13[1]:

We tried to make the change this past year,

but

there were too many other things happening here to achieve it.

But

come middle of 2008,

I am resolved that you will see a huge difference in the Purdue site.

It s been the default cytometry communication portal nowfor many, many years.

We have focused on good clean fun :bathbaby:with cytometry

quality, timely, simple - no spam.:3_8_14[1]:

Many people like that actually.
> The
> portal is almost overwhelming for us 22,000 daily page requests with
> over 2 Gigs daily download. In 2007 alone, downloads of 208,000
> powerpoint files, 233,000 PDF files, 8800 movies, 38,000 word
> document
> files. The education pages and the Cytometry Discussion Archive are
> the
> most hit for sure. Over 125,000 distinct files from our portal were
> accessed in 2007.

> But all good things must come to an end.

Come July 2008,

the usual Purdue web portal may well be no more.

It will be replaced with something entirely new.


Hopefully most will find it more useful

and


relevant - some will not like it.

Maybe we will be able to make everyone happy

....ha!..C'est la vie.:mad:


Some of you will be beta testers and advisers I hope.


So my best wishes to all in the cytometry field for 2008.

Regarding the past year on the discussion list, its been lively, with an average of 7 messages per day with 754 different individuals submitting at least one message. 139 messages had at least 6 responses. There were 1205 unique subject lines. Subscribers came from 64 top level domains. The usual bunch of suspects answered lots of messages and Marty Bigos seems to have too much time as he answered the most (thanks Marty!!).

Tragically, the second most prolific responder was Randy Fisher who passed away on December 5. Randy's responses were always short, to the point and accurate. It hurts to lose one of our own, particularly when it's one
of our most active members.

But that s the point isn't it. For many
years to come, we have the value of Randy's hundreds of suggestions over the years archived for the many new people who enter our field. Many of you probably never actually met Randy - but I bet most of you feel you knew him. One of the mysteries of the web I suppose. Our condolences to Randy's family - perhaps they didn't know how many people knew Randy "electronically" - but we all did.
You know we are a small field when
it comes to the big world of science so when we lose one person, the entire field morns.

To end 2007, let me make a big plug for a program we began at the 2006 ISAC congress.

Gary Durack from iCyt and myself started a small not- for-profit charity called

"Cytometry for Life" in response to Stephen
Lewis' compelling plea for some low cost CD4 devices. Our field
has done a lot of talking about this,

but

only a few people have really tried to do anything practical.

Well, folks we have all been doing cytometry for a very long time - it's time to do something.
"Cytometry for life" :mad:

(http://www.cytometryforlife.org)

is working hard. We have made tremendous progress in just one year. It would be great if you all decided to jump on board and play a small part.
You can give money, advice, moral support, talk to your politicians, community healthcare, charities,

whatever.

but get involved as be recognized as the cytometry community to solve this problem of bringing low cost, portable devices to the 65% or more of African's who don t live in the cities and towns where current CD4 technologies are available. Our goal is to work in areas not being served by current technologies.

We have heard these calls before, but folks we have to deal with this problem - it's your problem

if you call yourself a "cytometry" person.

Email me if you can help -

consider donating to the program, let's make it work. By the end of 2008,

I want to be telling you that the program is getting to
people who need this desperately. Help us achieve that for 2008.

I hope many of you got hold of a copy of our new double DVD set Cytometry 60 years of Innovation
if not ask your local rep from virtually any company in our field.

It might give you a good sense of
how strong the foundation in our field really is.
I will see many of you at the 2008 congress in Budapest. I know some of you think its going to be expensive so I took several hours myself and created a webpage for
the cheap ones out there so you have no excuses not to go...

(Cheap european Flights). :spy:

It's been a privilege to serve for the past 19 months as President of ISAC.

I will gladly pass that hat to Bob Murphy in May. ISAC is alive and well - membership is growing daily.

I would not be surprised to see us top 2000 by the end of the Congress in May.

I know that about 60% of the members of this list are NOT ISAC members.

Perhaps you should consider joining

the

Society that keeps many of you in business?:mad:

Welcome to the ISAC! - Mambo

> My best wishes for you all in 2008 from Purdue Paul
> --
> J. Paul Robinson
> SVM Professor of Cytomics
> Professor of Immunopharmacology & Biomedical Engineering
> Director, Purdue University Cytometry Laboratories
> President, International Society for Analytical Cytology
 
Fixing Papers on the PURDUE CYTOMETRY MAIL LIST your ISAC LEADERS!

From: Ray Hicks
Sent: Wednesday, October 17, 2001 8:42 PM
To: Cytometry Mailing List

 Subject: Re: Bad Flow Data & reviewing -- What can we do?:emot0:


Many good points Mario, :emot1:
but

I'm going to take you back a few years to our
discussion on dot plot versus contour, and how misleading contours are.:3_8_14[1]:
I'd
reverse your logic in " remember that contour plots are also
histograms (2D histograms), :mad:

and

they have no numbers on the "Z" axis
corresponding to event frequency. Why should univariate histograms have
them?", and suggest that contour plots need even more annotation.:D
I'm sorely tempted to attach a few figures to this e-mail, :rapture:

but

I've restrained myself, and made them available at::welcome:http://www.fcspress.com/seeWhatIMean.gif (Who's computer is this?)

and :drum:

http://www.fcspress.com/512AlongTheAxis.gif (Who's computer is this?)

The first <http://www.fcspress.com/seeWhatIMean.gif (Who's computer is this?) > shows how strikingly different contour plots of the same data can be

(the data is from the FlowJo tutorial set, the figures are made in FlowJo 3.2 and FCSPress 1.3).

The top left dotplot is from FlowJo,

and

shows the crowding you object to, the upper central plot is FlowJo's default contour plot of SSCvFSC with ten thousand
cells, the upper right plot is a plot of 1600 cells gated from the same
file
- doesn't look like fewer cells does it?:mad:
The lower left plot is a log 50% contour plot of the data in the top left
and top centre plots, what is one to make of those contours based on four
cells that jump out in the lower left?

The lower central plot is a dot plot from FCSPress, plotting data at 512 points along the axis

(the data has a range of 512 "channels"),

FCSPress has dithered the plot to represent how it would

(and does) print on a printer which isn't limited to screen resolution :caked:

(using the "clarify option),

you'll notice that using higher resolution avoids much of the coalescing to a black blob that you object to in dot plots

(the second figure, <http://www.fcspress.com/512AlongTheAxis.gif (Who's computer is this?) >,
.
shows this graph at full size with no dithering)
The lower right plot shows a density plot from FlowJo,
the smoothing belies the sparsity of the data.:suspicious::pout:

What's an expert to do when presented with this kind of thing?:faint:

Would labelling the upper left and lower left plots as having the same number of cells be enough to make you see them as representing the exact same data
set?

The dot plot of 1600 cells (not shown for brevity) clearly has fewer
cells than that of 10000, and does a better job of warning the viewer,
expert or not, of how confident they should be in making conclusions based
on the plot than numbering the events on the two contour plots (upper left
and upper right).
Oh, alright then, I've put a further figure up with two dot plots and two
contour plots with paired numbers of events at:
http://www.fcspress.com/nowDoYouSee.gif (Who's computer is this?)

The other issue I take is;

how is the collective going to select the
experts?Surely the people who are publishing this stuff ARE people

"with a modicum of experience in flow".

Putting the responsibility on editorial
boards is probably going to end up in a status quo.

How about pressuring your lab-fellows:mad:

 to sling the FACS aspect of papers, that

they're reviewing anyway,
:cool::confused:
 in your direction?
Ray

ps

as an aside,

there's something freaky :bathbaby: happening on the axes of these graphs -

they're 512 channel data,

but

the linear FSC axis runs out
just past 200,

and one of the events exceeds the maximum for side scatter:noidea:
(ie the one that juumps above the red line in the left hand plots -

has this beenfixed in later versions of

FlowJo? :scared::bolt:

Would this be

something an expert could

criticise/reject

a paper for?:mad:
 
How To Take Over Isac Congress To Force Fcs. 3.o Standard On The Corporations

From: Randy T. Fischer ([email protected])
Date: Thu Jul 17 1997 - 03:25:15 EST
* Next message: [email protected]: "LDL Receptor Assay for FH"
* Previous message: Bob Ashcroft: "RE: Cell Culture after DNA
Ploidy
Staining"
* Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
________________________________________
I agree with both

Marty and Gunter :3_9_7[1]:

in the very important issue

ofstandardizing data formatting.

I would point out that

lobbying ISAC:mad:

is only, however, part of the answer.

Regardless of what ISAC may choose to recommend,
it is still up to the

manufacturers to implement what they
want to do,

and

if they do not agree
with ISAC,

then too bad for ISAC

and

the flow community.

A potentially more powerful

force for change

might be the

FDA,
:emot0:
which regulates machines

used in CLINICAL settings.

If the

FDA
:mad:
could be persuaded


to

requireall

CLINICAL data be universally

both
accessible and readable,
then the manufacurers

would be forced
:mad:

to upgrade machines

and

software or lose
:mad:

the
LUCRATIVE CLINICAL market.:mad:

This would make anlyzing data from different sources easier, and could facilitate the exchange

ofcrucial clinical results

from various trials where multiple sites

and

machines are in use.:3_8_14[1]:

So how does this get done?:3_9_7[1]:

Gunter ;)

(and Paul's agreeing response):mad:

 are correct this needs to be revisited at Asilomar,

with perhaps an additional idea.

Any concrete standardization protocol,

FCS3.0

or

whatever

it ends up being designated,

should be then presented to any

and

all regulatory agencies

by

ISAC

to ensure

no

individual manufacturer decides

FCS3.0 in their format is acceptable,
even

if it is not universally readable.

Randy T. Fischer
NIA/NIH
GRC
Baltimore, MD 21224
[email protected]




NOW

PAUL AND THE CREW RUN ISAC
:mad:
THE DEVELOPERS FORM THE MAIL HAVE TAKEN CONTROL!

They are only

THE

FDA
:mad:
AWAY FROM TOTAL CONTROL OF THE MARKET!:mad:

look at all these small companies:mad:
trying to stick there nose in our field!

THE COALITION FOR CHANGE!:mad:
 
Control Of Isac Congress

GOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOGLE
PURDUE CYTOMETRY MAIL LIST


READ HOW THEY GREW AND TOOK CONTROL OF ISAC CONGRESS!

FILTERING OUT THE LIST!

DEVELOPERS OF SOFTWARE

MACHINES

CODE

THERE MUST BE CHANGE


:mad:


 
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